CORE--TISSUE CULTURE, MICROSCOPY-IMAGING FACILITY

核心——组织培养、显微镜成像设备

• 批准号: 6849085
• 负责人: Miriam S Domowicz
• 金额: $ 25.02万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6849085
• 关键词: biomedical facility; cell biology; developmental neurobiology; gel electrophoresis; imaging /visualization /scanning; microscopy; tissue /cell culture

DESCRIPTION (provided by applicant): The Administrative and Scientific Core functions as a centralized unit to provide services to individual projects. The administrative component of the core is responsible for managing the finances of the program, including grant preparation, non competing renewals and reports, organizing program activities, including seminars, journal clubs and the Advisory Board consultations, and preparation of publications relevant to the program. The tissue culture component of the core provides centralized media preparation, supplies common cell cultures, and stock cell lines and some monoclonal antibodies in an efficient, coordinated and cost-saving manner. The microscopy/imaging facility provides investigators the opportunity to capture images from gels (DNA or protein) with built-in parameters for ethidium bromide, SYBR green, SYPRO orange, and Coomassie blue. Once the image has been captured it is available for adjustment, analysis or printing. The physical components in this facility (300 square feet) include an inverted microscope with fluorescence capabilities, optics and filters, a z-focus motor, 3-chip video system, and software that provides for macro editing and 3D processing. Over the next project period a tissue preparation/in situ facility will be developed for program investigators. These techniques are increasingly being used by Projects I, II and III, and each investigator is currently borrowing time on other investigator's equipment at a distance from their own laboratories. Because all projects use embryonic chick tissue for mRNA in situ hybridization analysis, a library of chick specific cDNAs is being shared among the investigators; most of them were constructed in their own laboratories. A continuous effort will be made for further expansion of this component of the core as needed.

描述（由申请人提供）：行政和科学核心的职能是 集中单位为个别项目提供服务。核心的行政部分负责管理该计划的财务，包括赠款准备、非竞争性更新和报告、组织计划活动，包括研讨会、期刊俱乐部和咨询委员会咨询，以及准备与该计划相关的出版物。核心的组织培养组件以高效、协调和节省成本的方式提供集中的培养基制备，提供常见的细胞培养物、储备细胞系和一些单克隆抗体。显微镜/成像设备为研究人员提供了从具有溴化乙锭、SYBR 绿、SYPRO 橙和考马斯蓝内置参数的凝胶（DNA 或蛋白质）捕获图像的机会。捕获图像后，即可进行调整、分析或打印。该设施（300 平方英尺）的物理组件包括具有荧光功能的倒置显微镜、光学器件和滤光片、z 聚焦电机、3 芯片视频系统以及提供宏编辑和 3D 处理的软件。在下一个项目期间，将为项目研究人员开发组织制备/原位设施。这些技术越来越多地被项目 I、II 和 III 使用，每个研究人员目前都在远离自己实验室的其他研究人员的设备上借用时间。因为所有项目都使用胚胎鸡组织来获取 mRNA 原位杂交分析，研究人员正在共享鸡特异性 cDNA 文库；其中大多数是在他们自己的实验室中建造的。将根据需要不断努力进一步扩展核心的这一组成部分。