Proteomics Core

蛋白质组学核心

• 批准号: 7185716
• 负责人: Joseph A Loo
• 金额: $ 17.53万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7185716
• 关键词: air pollution; airborne allergen; asthma; bioinformatics; biomedical facility; biotechnology; engine exhaust; inflammation; mass spectrometry; monocyte; oxidative stress; pollution related respiratory disorder; posttranslational modifications; proteomics; respiratory hypersensitivity; two dimensional gel electrophoresis

The Proteomics Core supports the research goals of the Asthma Allergic Disease Research Center (AADRC) by providing state-of-the-art mass spectrometry and expertise for the characterization of proteins and their post-translational modifications such as phosphorylation, and profiling changes in protein expression. The AADRC is involved in studying the adjuvant effects of diesel exhaust particles (DEP) and concentrated air particles in allergic airway inflammation and asthma. The Core, centrally located for UCLA researchers in the Molecular Sciences Building, houses a wide range of equipment for two dimensional gel electrophoresis protein separation, mass spectrometry-based protein characterization, and software and bioinformatics to facilitate protein identification. The Proteomics Core, under the leadership of Dr Joseph Loo, will facilitate the proteome analysis on: (i) bronchoalveolar lavage fluid with a view to demonstrating PM-induced oxidative stress responses and the role of phase II enzymes in allergic inflammation, (ii) characterize the proteome of human nasal lavage fluid to measure PM-induced oxidative stress responses in human subjects with high and low responses to DEP and to measure responses from subjects showing potent sulforaphane-induced protection from DEP and cat allergen exposure, and (iii) perform proteome analysis on human peripheral blood monocytes to confirm the genetic response differences between the high and low responder subsets. Proteomic approaches will be applied to develop a multi-pronged strategy to assemble the most complete picture of the cellular, molecular, and biochemical mechanisms of particular pollution enhancement of allergic inflammation. Relevance: The Proteomics Core houses the necessary technology to monitor the changes in protein expression measured in body fluids, tissues, and cells that result from exposure to particulate pollution. The goal of the Core is to find the molecular and biochemical link between the adverse health effects of particulate pollution and allergic inflammation.

蛋白质组学核心支持哮喘过敏疾病研究中心的研究目标 （AADRC）通过提供最先进的质谱和专业知识来表征蛋白质 及其翻译后修饰，例如磷酸化以及蛋白质的分析变化 表达。 AADRC参与研究柴油排气颗粒（DEP）和 过敏性气道炎症和哮喘中的浓缩空气颗粒。核心位于加州大学洛杉矶分校 分子科学大楼的研究人员，设有二维凝胶的广泛设备 电泳蛋白分离，基于质谱的蛋白质表征和软件和 生物信息学以促进蛋白质鉴定。蛋白质组学核心，在约瑟夫博士的领导下 loo，将促进蛋白质组分析：（i）支气管肺泡灌洗液，以证明 PM诱导的氧化应激反应以及II期酶在过敏性炎症中的作用，（II） 表征人类鼻腔灌洗液的蛋白质组，以测量PM诱导的氧化应激反应 对DEP的反应高和低反应的人类受试者，并衡量显示的受试者的反应 有效的硫烷诱导的防止PEP和CAT过敏原暴露的保护，（iii）执行蛋白质组 分析人类外周血单核细胞以确认遗传反应差异 高响应者子集。蛋白质组学方法将用于制定多管齐下的策略 组装特定的细胞，分子和生化机制的最完整图像 过敏性炎症的污染增强。 相关性：蛋白质组学核心容纳必要的技术来监测蛋白质的变化 暴露于颗粒污染的体液，组织和细胞中测量的表达。这 核心的目标是找到分子和生化联系 颗粒污染和过敏性炎症。