Chemical Approaches to the Study of Cell Division

研究细胞分裂的化学方法

• 批准号: 6868209
• 负责人: Gregory P Tochtrop
• 金额: $ 4.99万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868209
• 关键词: SDS polyacrylamide gel electrophoresis; Xenopus oocyte; affinity chromatography; analytical chemistry; biochemistry; cell cycle; cell growth regulation; chemical structure function; chemical synthesis; cyclins; genetic screening; ion exchange chromatography; method development; postdoctoral investigator; protein transport; proteolysis; western blottings

DESCRIPTION (provided by applicant): The broader goals of the proposed work are aimed at the development of chemical approaches to the study of cell division and ultimately cancer. More specifically, the developing field of 'chemical genetics' will be applied to the understanding of cell cycle regulators and the mitotic spindle checkpoint. The King laboratory has identified several compounds that block exit from mitosis and inhibit cyclin proteolysis. The goals of the proposed research are to identify the biochemical targets of these compounds and pursue the mechanism of how these compounds inhibit cyclin proteolysis. Additionally, we propose to develop a new strategy that will enable the rapid identification of protein targets of active compounds found in chemical genetic screens.

描述（由申请人提供）： 拟议工作的更广泛目标是开发化学方法来研究细胞分裂并最终研究癌症。更具体地说，“化学遗传学”的发展领域将应用于理解细胞周期调节因子和有丝分裂纺锤体检查点。 King 实验室已鉴定出几种可阻止有丝分裂退出并抑制细胞周期蛋白水解的化合物。拟议研究的目标是确定这些化合物的生化靶点，并探究这些化合物如何抑制细胞周期蛋白水解的机制。此外，我们建议开发一种新策略，能够快速识别化学遗传筛选中发现的活性化合物的蛋白质靶标。