Characterization of novel core promoter elements

新型核心启动子元件的表征

• 批准号: 7053624
• 负责人: Timur Yusufzai
• 金额: $ 4.6万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7053624
• 关键词: CpG islands; DNA directed RNA polymerase; Drosophilidae; RNA interference; binding sites; chromatin immunoprecipitation; gene expression; genetic promoter element; genetic transcription; postdoctoral investigator; protein protein interaction; transcription factor

DESCRIPTION (provided by applicant): This proposal focuses on the understanding of core promoters. The most recent core promoter element identified, the motif ten element (MTE), is located downstream of the transcription start site. A preliminary study has found that transcription by the MTE requires a specific factor (or factors) that is not required for TATA-dependent transcription. These factors are likely to be novel basal transcription factors. This Research Proposal provides an outline for a systematic study of the MTE and its required factors. In addition, the initiation of a project involving the characterization of CpG islands as core promoters is proposed. Specifically, (1) to purify and characterize the factors that mediate MTE-dependent transcription, ones likely to be novel basal transcription factors; (2) investigate the interaction of TFIID with the MTE; (3) investigate the sequences within the CpG islands that have core promoter activity. Together, these studies will contribute to the understanding of core promoters and a fundamental cellular process- transcription activation.

描述（由申请人提供）：该提案的重点是对核心促进者的理解。确定的最新核心启动子元素，即Motif Ten Element（MTE），位于转录起始位点的下游。一项初步研究发现，MTE的转录需要一个特定因素（或因子），而TATA依赖性转录不需要。这些因素可能是新的基础转录因子。该研究建议为对MTE的系统研究及其所需因素提供了概述。此外，提出了涉及将CpG岛作为核心启动子表征的项目的启动。具体而言，（1）净化和表征介导MTE依赖性转录的因素，这可能是新的基础转录因子； （2）研究TFIID与MTE的相互作用； （3）研究具有核心启动子活性的CpG岛内序列。总之，这些研究将有助于理解核心启动子和基本的细胞过程转录激活。