Characterization of novel core promoter elements

新型核心启动子元件的表征

基本信息

批准号：
7171853
负责人：
Timur Yusufzai
金额：
$ 4.88万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-01 至 2008-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7171853
关键词：
Affect Be++ element Beryllium Binding Cell physiology Classification CpG Islands Elements Gene Expression General Transcription Factors Genes Genetic Transcription Goals Human Indium Mediating Nucleotides Proteins RNA Polymerase II Research Proposals Role Site TATA Box Testing Transcription Factor TFIID Transcription Initiation Transcription Initiation Site Transcriptional Activation Transcriptional Regulation Work insight novel promoter

Affect Be++ element Beryllium Binding Cell physiology Classification CpG Islands Elements Gene Expression General Transcription Factors Genes Genetic Transcription Goals Human Indium Mediating Nucleotides Proteins RNA Polymerase II Research Proposals Role Site TATA Box Testing Transcription Factor TFIID Transcription Initiation Transcription Initiation Site Transcriptional Activation Transcriptional Regulation Work insight novel promoter

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项目摘要

DESCRIPTION (provided by applicant): This proposal focuses on the understanding of core promoters. The most recent core promoter element identified, the motif ten element (MTE), is located downstream of the transcription start site. A preliminary study has found that transcription by the MTE requires a specific factor (or factors) that is not required for TATA-dependent transcription. These factors are likely to be novel basal transcription factors. This Research Proposal provides an outline for a systematic study of the MTE and its required factors. In addition, the initiation of a project involving the characterization of CpG islands as core promoters is proposed. Specifically, (1) to purify and characterize the factors that mediate MTE-dependent transcription, ones likely to be novel basal transcription factors; (2) investigate the interaction of TFIID with the MTE; (3) investigate the sequences within the CpG islands that have core promoter activity. Together, these studies will contribute to the understanding of core promoters and a fundamental cellular process- transcription activation.

描述（由申请人提供）：该提案的重点是对核心促进者的理解。确定的最新核心启动子元素，即Motif Ten Element（MTE），位于转录起始位点的下游。一项初步研究发现，MTE的转录需要一个特定因素（或因子），而TATA依赖性转录不需要。这些因素可能是新的基础转录因子。该研究建议为对MTE的系统研究及其所需因素提供了概述。此外，提出了涉及将CpG岛作为核心启动子表征的项目的启动。具体而言，（1）净化和表征介导MTE依赖性转录的因素，这可能是新的基础转录因子；（2）研究TFIID与MTE的相互作用；（3）研究具有核心启动子活性的CpG岛内序列。总之，这些研究将有助于理解核心启动子和基本的细胞过程转录激活。