Development of 13C-retinol isotope dilution techniques

13C-视黄醇同位素稀释技术的开发

基本信息

批准号：
7060827
负责人：
SHERRY A TANUMIHARDJO
金额：
$ 12.93万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-08-01 至 2009-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Improved, simplified methods to determine vitamin A status are needed to accurately assess both individuals and populations at risk of either vitamin A deficiency or toxicity. The vitamin A statuses of women and children in lower socioeconomic groups in both the underdeveloped world and the United States are compromised. Multiple reasons for this compromised status include low dietary intakes, increased demands during pregnancy and lactation and recurrent infections. The 13C-retinol isotope dilution (13C-RID) test, first developed in the rodent model using 13C4-retinyl acetate and gas chromatography-combustion-isotope ratio mass spectrometry (GCCIRMS), is sensitive and accurate. The major goals of this project are to validate the 13C-RID test for use in human population groups to assess total body reserves of vitamin A and determine vitamin A requirements of young adults. The success of this project will provide a sensitive tool for investigators to evaluate the vitamin A status of individuals and population groups using minimal sample sizes. The test has direct application to determining the outcome of intervention strategies aimed at improving vitamin A status and in determining vitamin A needs of humans and animals throughout their life cycles. The proposed studies will for the first time: (1) validate the 13C-RID test to assess a sub-toxic vitamin A status in non-human primates; (2) validate the 13C-RID test to assess sub-clinical vitamin A deficiency in the weanling pig; (3) refine the 13C-RID test for use in humans by looking at different dose levels and blood sampling times; and (4) investigate the use of the 13C-RID test in a human intervention study designed to look at vitamin A requirements in young adults.

描述（由申请人提供）：需要改进的简化方法来确定维生素A状态，以准确评估具有维生素A缺乏症或毒性风险的个人和人群。在欠发达世界和美国，妇女和儿童的维生素A地位受到妥协。这种损害状态的多种原因包括饮食中的摄入量低，怀孕期间的需求增加，以及反复感染。 13C-元素同位素稀释（13C-RID）测试是在啮齿动物模型中首次使用13c4-乙二醇和气相色谱 - 燃烧 - 同位素比率质谱法（GCCIRNS）开发的，是敏感且准确的。该项目的主要目标是验证13C-RID测试在人口组中使用，以评估维生素A的全身储量并确定年轻人的维生素A要求。该项目的成功将为研究人员提供一种敏感的工具，以使用最小的样本量评估个人和人群的维生素A状态。该测试直接应用于确定旨在改善维生素A状态并确定人类和动物的维生素A的需求的干预策略的结果。拟议的研究将首次：（1）验证13C-RID检验以评估非人类灵长类动物中的亚毒性维生素A状态；（2）验证13C-RID测试以评估断奶猪中的亚临床维生素A缺乏；（3）通过查看不同的剂量水平和血液采样时间来完善13C-RID测试以在人类中使用；（4）研究在人类干预研究中使用13C-RID测试的使用，旨在研究年轻人的维生素A需求。