Catalytic DNA Biosensor for Toxic Metal Ions

用于检测有毒金属离子的催化 DNA 生物传感器

基本信息

批准号：
6993035
负责人：
Yi Lu
金额：
$ 10万
依托单位：
DZYMETECH, INC.
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-05 至 2006-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6993035
关键词：
DNA air sampling /monitoring bioengineering /biomedical engineering biohazard detection biomedical equipment development biosensor device cadmium catalyst clinical biomedical equipment colorimetry fluorescence heavy metals lead mercury portable biomedical equipment

项目摘要

DESCRIPTION (provided by applicant): A general method of developing catalytic DNA biosensors for on-site, real-time, and cost-effective detection and quantification of multiple heavy metal ions, such as mercury, cadmium and lead, simultaneously will be explored. Exposure to these metal ions can bring severe health effects to human beings, such as damages to brain and kidneys, and cancers. They can also cause adverse effects on neural and brain development in children and the effects in children are often irreversible. Therefore there is an urgent need of portable sensors for these metal ions for household uses, for clinical applications in rural or remote areas, and for first-responders in the war against terrorism, where on-site, real-time, and cost-effective detection and quantification are critical. This Phase I application addresses critical issues of how to apply a successful method in designing sensors for one metal ion to those of other metal ions, and how to enhance selectivity of those sensors. It is built upon a recent success in the PI's lab where highly sensitive and selective fluorescent and colorimetric sensors for lead have been demonstrated based on catalytic DMA. In phase I, the feasibility of applying this method as the general protocol to the two other heavy metals, namely mercury and cadmium, will be investigated. Specifically, a combinatorial biology technique called in vitro selection will be carried out to select Hg(ll)- and Cd(ll)-specific catalytic DMA. After negative selection strategies are used to improve the selectivity of the selected DNA molecules, fluorescent and colorimetric sensors for mercury and cadmium will be constructed and tested by attaching fluorophore/quencher and gold nanoparticles, respectively, to the catalytic DNA molecules. After achieving these goals in Phase I, catalytic DNA specific for arsenic and chromium will be selected in Phase II and the prototype sensor kits for these heavy metal ions will be developed for testing and marketing the sensors in environmental monitoring and medical diagnostic applications.

描述（由申请人提供）：将探索一种开发催化 DNA 生物传感器的通用方法，用于同时现场、实时、经济高效地检测和定量多种重金属离子，如汞、镉和铅。接触这些金属离子会给人类带来严重的健康影响，例如对大脑和肾脏的损害以及癌症。它们还会对儿童的神经和大脑发育造成不利影响，而且对儿童的影响往往是不可逆转的。因此，迫切需要针对这些金属离子的便携式传感器，用于家庭使用、农村或偏远地区的临床应用以及反恐战争中的急救人员，这些传感器可以现场、实时且具有成本效益。检测和定量至关重要。该第一阶段的应用解决了如何应用成功的方法来设计一种金属离子传感器到其他金属离子传感器的关键问题，以及如何提高这些传感器的选择性。它建立在 PI 实验室最近取得的成功基础上，该实验室基于催化 DMA 展示了高灵敏度和选择性的铅荧光和比色传感器。在第一阶段，将研究将此方法作为一般方案应用于其他两种重金属（即汞和镉）的可行性。具体来说，将进行一种称为体外选择的组合生物学技术来选择 Hg(II) 和 Cd(II) 特异性催化 DMA。使用负选择策略来提高所选 DNA 分子的选择性后，将通过将荧光团/猝灭剂和金纳米颗粒分别附着到催化 DNA 分子上来构建和测试汞和镉的荧光和比色传感器。在第一阶段实现这些目标后，第二阶段将选择针对砷和铬的催化 DNA，并将开发针对这些重金属离子的原型传感器套件，用于在环境监测和医疗诊断应用中测试和营销传感器。