A National Resource for Postmortem Brain Research

国家死后脑研究资源

• 批准号: 7119234
• 负责人: FRANCINE M. BENES
• 金额: $ 131.8万
• 依托单位: MCLEAN HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-17 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7119234
• 关键词: Internet; brain; clinical research; computer human interaction; computer program /software; computer system hardware; data collection methodology /evaluation; degenerative motor system disease; dementia; gene expression; genetic polymorphism; health science research; human tissue; microarray technology; molecular biology information system; postmortem; psychosis; tissue /cell preparation; tissue resource /registry

DESCRIPTION (provided by applicant): This is a competitive renewal application for the Harvard Brain Tissue Resource Center, a national resource for the acquisition, processing, storage and distribution of high quality postmortem (PM) brain to the neuroscience community. This facility collects brains from normal controls (CON), as well as individuals with a variety of movement disorders (Huntington's chorea and Parkinson's disease), dementias (Alzheimer's disease, frontotemporal dementias) and major psychoses (schizophrenia and bipolar disorders). The HBTRC distributes tissue specimens to neuroanatomists, neuropathologists, neuropharmacologists, neurochemists and molecular biologists throughout the U.S. Over the past 5 years, this tissue has contributed to more than 170 scientific publications. During the current funding period, the HBTRC has undergone a major restructuring that has resulted in an increase in the overall quality and efficiency of its operation. An important outcome of this re-organization has been an increase in the number of fresh, frozen cases and a marked decrease in the postmortem interval (PMI). As a result of this effort, abundant, high quality tissue is now available for a broad array of state-of-the-art research applications. The number of CONs received by the HBTRC has more than doubled during the current funding period and the HBTRC is now able to provide investigators with sets of CONs and diseased brains matched for age, PMI, gender and hemisphere. For the major psychoses, where the acquisition of postmortem brains is actually challenging, the number of schizophrenic and bipolar cases has also increased. Since these are largely from community-based referrals, the potential confounding effects of institutionalization, inanition and substance abuse are relatively lower than in medical examiner cases. To facilitate the donation process, the HBTRC has established a website with a) Informed Consent forms for potential donors and their families, b) instructions for handling brains by pathologists or dieners involved in brain removal, and c) application forms for investigators seeking tissue for their research. The HBTRC has also developed a User-Interactive Database, so that investigators who receive tissue from the "bank" can obtain demographics, neuropathology reports and images of gross dissections and histological slides for their cases. With supplemental funding from NIH, the HBTRC is now obtaining gene expression profiling and SNP analyses using the standard Affymetrix microarray system and this information will be immediately placed in our public domain National Databank as it is obtained. All investigators receiving tissue from the HBTRC will also be encouraged to "deposit" their findings in the Databank so that they will be available to the neuroscience community for complex relational analyses. Over the next funding period, these new initiatives will enhance the functioning of the "brain bank" in its role as a vital national resource.

描述（由申请人提供）：这是哈佛大学脑组织资源中心的竞争更新应用，这是一种为神经科学界的高质量验尸（PM）大脑的获取，加工，储存和分配的国家资源。 该设施从正常对照组（CON）以及患有多种运动障碍（亨廷顿的唱片和帕金森氏病），痴呆症（阿尔茨海默氏病，额叶痴呆症）和主要精神病（Schizizophrenia and Bicarrenia and Biolar灾难）的个体中收集大脑。 HBTRC将组织标本分配给神经解剖学家，神经病理学家，神经药物学家，神经化学家和分子生物学家在过去的5年中，这种组织为170多个科学出版物做出了贡献。在当前的资金期间，HBTRC进行了重大重组，导致其运营的总体质量和效率提高。这种重组的重要结果是，新鲜，冷冻病例的数量增加，验尸间隔（PMI）显着减少。由于这一努力，现在可用于广泛的最先进的研究应用程序。 HBTRC收到的缺点在当前的资金期间增加了一倍以上，HBTRC现在能够为调查人员提供与年龄，PMI，性别和半球相匹配的缺点和患病的大脑的集合。对于主要的精神病而言，在验收后大脑实际上具有挑战性，精神分裂症和双极病例的数量也有所增加。由于这些主要来自基于社区的推荐，因此制度化的潜在混杂影响，侵害和滥用药物的效果相对较低，低于体检医师案件。为了促进捐赠过程，HBTRC建立了一个网站，其网站a）为潜在的捐助者及其家人提供知情同意书，b）涉及大脑清除的病理学家或士兵处理大脑的指示，c）c）针对寻求组织进行研究的调查人员的申请表。 HBTRC还开发了一个用户相互作用的数据库，因此从“银行”中接收组织的研究人员可以获取人口统计学，神经病理学报告以及大剖析图像和案例的组织学幻灯片的图像。通过NIH的补充资金，HBTRC现在使用标准Affymetrix微阵列系统获得基因表达分析和SNP分析，并且该信息将立即放置在我们获得的公共领域国家数据库中。也鼓励所有从HBTRC接收组织的研究人员将其发现“存入”数据库中，以便将其向神经科学界可用，以进行复杂的关系分析。在下一个融资期内，这些新计划将增强“大脑银行”作为重要的国家资源的作用。