CORE--CELL

核心--细胞

• 批准号: 6924192
• 负责人: ELIZABETH M. JAFFEE
• 金额: $ 6.59万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6924192
• 关键词: biomedical facility; cancer registry /resource; cell bank /registry; cell line; cryopreservation; neoplasm /cancer vaccine; neoplastic cell; tissue /cell culture

We are proposing to have a central Cell CORE for expanding, cryopreserving, and distributing tumor and vaccine cells that will be utilized by alt 4 projects in this NCDDG program. The main purpose of this CORE is to provide standardized reagents to all 4 projects in the NCDDG program. This will allow for comparative studies since cell lines will be used at equivalent passages, and will have undergone regular stability testing. The in vivo feasibility and optimization data obtained using these Core generated reagents will be suitable for including in investigator-initiated INDs submitted to support the clinical trials that arise from this NCDDG program. Cell banks for vaccine cell lines and tumor lines (mouse and human) will be produced under standard operating procedures, cryopreserved, and distributed to investigators in the program as needed. Quality control assays will be established for each banked reagent and performed on the expanded bank of reagents to insure the stability of the reagent has been maintained. Dr. Jaffee will be the CORE leader. The CORE will be responsible for distributing the reagents upon request from an investigator. The CORE will also be responsible for emonstrating stability of the reagents. Individual investigators will be responsible for limited expansion of the cell lines received from the CORE that are used in a particular experiment. There are four specific aims of this CORE. Specific aim #1. Produce, cryopreserve, and distribute to investigators, Master Cell Banks (MCB) of individual cell lines. Specific aim #2. Perform quality control assays on the pre-expansion and post-expansion (MCB) cell lines. Specific aim #3. Perform yearly stability testing of MCB cell lines. Specific aim #4. Distribute cell lines to all four investigators.

我们建议在此NCDDG程序中使用Alt 4项目利用的中央细胞核，用于扩展，冷冻水平和分发肿瘤和疫苗细胞。该核心的主要目的是为NCDDG计划中的所有4个项目提供标准化试剂。这将允许进行比较研究，因为细胞系将在同等通道下使用，并将进行常规稳定性测试。使用这些核心生成的试剂获得的体内可行性和优化数据将适合于提交的研究者发起的IND，以支持该NCDDG产生的临床试验 程序。疫苗细胞系和肿瘤系（小鼠和人）的细胞库将根据标准操作程序产生，冷冻保存并根据需要分配给研究人员。将为每种库试剂建立质量控制测定法，并在扩大的试剂库进行，以确保已维持试剂的稳定性。贾菲博士将成为核心领导者。核心将根据调查人员的要求负责分发试剂。核心还将负责提出试剂的稳定性。单个研究人员将负责特定实验中使用的核心收到的细胞系的有限膨胀。该核心有四个特定的目标。特定目标＃1。生产，冷冻水果和分发给研究人员，单个细胞系的大型细胞库（MCB）。特定目标＃2。对膨胀前和膨胀后（MCB）细胞系进行质量控制测定。特定目标＃3。对MCB细胞系进行年度稳定性测试。特定目标＃4。将细胞系分发给所有四个研究者。