CORE--CELL

核心--细胞

• 批准号: 6924192
• 负责人: ELIZABETH M. JAFFEE
• 金额: $ 6.59万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6924192
• 关键词: biomedical facility; cancer registry /resource; cell bank /registry; cell line; cryopreservation; neoplasm /cancer vaccine; neoplastic cell; tissue /cell culture

We are proposing to have a central Cell CORE for expanding, cryopreserving, and distributing tumor and vaccine cells that will be utilized by alt 4 projects in this NCDDG program. The main purpose of this CORE is to provide standardized reagents to all 4 projects in the NCDDG program. This will allow for comparative studies since cell lines will be used at equivalent passages, and will have undergone regular stability testing. The in vivo feasibility and optimization data obtained using these Core generated reagents will be suitable for including in investigator-initiated INDs submitted to support the clinical trials that arise from this NCDDG program. Cell banks for vaccine cell lines and tumor lines (mouse and human) will be produced under standard operating procedures, cryopreserved, and distributed to investigators in the program as needed. Quality control assays will be established for each banked reagent and performed on the expanded bank of reagents to insure the stability of the reagent has been maintained. Dr. Jaffee will be the CORE leader. The CORE will be responsible for distributing the reagents upon request from an investigator. The CORE will also be responsible for emonstrating stability of the reagents. Individual investigators will be responsible for limited expansion of the cell lines received from the CORE that are used in a particular experiment. There are four specific aims of this CORE. Specific aim #1. Produce, cryopreserve, and distribute to investigators, Master Cell Banks (MCB) of individual cell lines. Specific aim #2. Perform quality control assays on the pre-expansion and post-expansion (MCB) cell lines. Specific aim #3. Perform yearly stability testing of MCB cell lines. Specific aim #4. Distribute cell lines to all four investigators.

我们提议建立一个中央 Cell CORE，用于扩展、冷冻保存和分配肿瘤和疫苗细胞，该细胞将由该 NCDDG 计划中的替代 4 项目使用。该 CORE 的主要目的是为 NCDDG 计划中的所有 4 个项目提供标准化试剂。这将允许进行比较研究，因为细胞系将在同等传代中使用，并且将进行定期稳定性测试。使用这些核心生成的试剂获得的体内可行性和优化数据将适合包含在研究者发起的 IND 提交中，以支持由该 NCDDG 产生的临床试验 程序。疫苗细胞系和肿瘤细胞系（小鼠和人类）的细胞库将在标准操作程序下生产、冷冻保存，并根据需要分发给项目中的研究人员。将为每个库存试剂建立质量控制测定，并在扩大的试剂库上进行，以确保保持试剂的稳定性。 Jaffee 博士将担任 CORE 领导者。 CORE 将负责根据研究者的要求分发试剂。 CORE 还将负责证明试剂的稳定性。个别研究人员将负责对从 CORE 接收的用于特定实验的细胞系进行有限的扩增。该核心有四个具体目标。具体目标#1。生产、冷冻保存单个细胞系的主细胞库 (MCB) 并分发给研究人员。具体目标#2。对扩增前和扩增后 (MCB) 细胞系进行质量控制测定。具体目标#3。每年对 MCB 细胞系进行稳定性测试。具体目标#4。将细胞系分发给所有四位研究人员。