Charting colorectal cancer Interactomes at structural resolution using chemical crosslinking and mass spectrometry

使用化学交联和质谱以结构分辨率绘制结直肠癌相互作用组图

• 批准号: 2748301
• 金额: --
• 依托单位: Institute of Cancer Research
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2748301
• 关键词: Charting; colorectal; cancer; Interactomes; structural

Large numbers of cancer mutations have been exposed through genomics; however, it remains unclear how most cancer genes function and exert influence on disease processes. Here we propose to develop a quantitative approach to map protein interactions on global scale at high throughput using cross-linking mass spectrometry (XL-MS). The method will be used to chart the native protein interaction landscapes across cancer cell lines, generating structural information on protein topology and protein-protein interaction (PPI) surfaces. A deeper understanding of how somatic mutations affect protein structure, function and rewiring of cellular networks, will provide mechanistic insights and underpins new intervention strategies.

基因组学揭示了大量癌症突变；然而，目前尚不清楚大多数癌症基因如何发挥作用并对疾病过程产生影响。在这里，我们建议开发一种定量方法，使用交联质谱 (XL-MS) 以高通量绘制全球范围内的蛋白质相互作用图谱。该方法将用于绘制跨癌细胞系的天然蛋白质相互作用图谱，生成蛋白质拓扑和蛋白质-蛋白质相互作用（PPI）表面的结构信息。更深入地了解体细胞突变如何影响蛋白质结构、功能和细胞网络的重新布线，将提供机制见解并支撑新的干预策略。