Cell and Molecular Biology of Marine Elasmobranchs

海洋软骨鱼的细胞和分子生物学

• 批准号: 7073488
• 负责人: DAVID William BARNES
• 金额: $ 35.7万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7073488
• 关键词: Chondrichthyes; biological models; brain cell; cell biology; cell line; cell proliferation; embryo /fetus tissue /cell culture; gene expression; kidney cell; liver; model design /development; molecular biology; technology /technique development; transfection /expression vector

DESCRIPTION (provided by applicant): This proposal responds to the Program "Development of Non-mammalian Models and Related Biological Materials for Research." Specifically, the program announcement mentions "Species such as fishes...and cell lines and cell culture models for non-mammalian animals where culture systems are not well developed but would be widely used." We propose to develop cell lines from marine elasmobranchs (sharks and skates), animals that are broadly exploited in biomedical research as models in physiology, endocrinology, toxicology and genomics. Currently there are no homologous in vitro systems by which investigators using these models can test hypotheses or confirm predictions at a molecular level, and application of new technologies is needed. The premise driving this project is that in vitro culture of specialized cell types can be accomplished by taking non-conventional approaches, including removal or reduction of serurnas a medium component and replacement with cell type-specific supplements, such as peptide growth factors. We have used this approach to generate cell lines from zebrafish, pufferfish (fugu), and other fish species. This work will take advantage of the unique resources of the Mount Desert Island Biological Laboratory, a research facility dedicated to the study of marine organisms to discover biological mechanisms of significance to human health, and world center for the use of the dogfish shark (Squalus acanthias) and little skate (Raja erinacea) as research models. We propose to develop differentiation-competent, continuously proliferating cell lines from (1) liver, brain, kidney and salt (rectal) gland of the dogfish shark, and (2) liver and embryo of the little skate. We also propose to (3) develop systems for DNA transfection and plasmid-derived gene expression in these cell lines. Success will provide a platform for original and innovative experiments, accelerating the development of concepts driving multiple areas of biomedical research addressed by the large number of investigators using these models.

描述（由申请人提供）：该提案响应“非哺乳动物模型的开发以及相关的研究生物材料”。具体而言，该计划公告提到“诸如鱼类等物种以及非哺乳动物动物的细胞系和细胞培养模型，那里的培养系统尚未得到很好的发展，但将被广泛使用”。我们建议从海洋弹性分支（鲨鱼和溜冰鞋）中开发细胞系，这些动物在生物医学研究中被广泛利用为生理学，内分泌学，毒理学和基因组学模型。当前，没有同源的体外系统，使用这些模型的研究人员可以在分子水平上检验假设或确认预测，并且需要新技术的应用。推动该项目的前提是，可以通过采用非规定方法来实现专业细胞类型的体外培养，包括去除或减少中纳斯成分，并用细胞类型特异性补充剂（例如肽生长因子）代替。我们已经使用了这种方法来产生斑马鱼，河豚（Fugu）和其他鱼类的细胞系。这项工作将利用山山岛生物实验室的独特资源，该研究机构致力于研究海洋生物，以发现对人类健康有重要意义的生物学机制，以及使用Dogfish Shark（Squalus acanthias）使用世界中心（Raja Erinacea）（Raja Erinacea）（Raja Erinacea）。我们建议从（1）（1）dog鱼鲨鱼的肝脏，脑，肾脏和盐（直肠）腺体以及（2）小滑板的肝脏和胚胎的肝脏，脑，肾脏和盐（直肠）腺体开发出差异化的，连续增殖的细胞系。我们还建议（3）在这些细胞系中开发用于DNA转染和质粒衍生的基因表达的系统。成功将为原始和创新的实验提供一个平台，加速了使用这些模型来推动大量研究人员解决生物医学研究的概念的发展。