NATIONAL STEM CELL RESOURCE

国家干细胞资源

• 批准号: 6256538
• 负责人: DAVID William BARNES
• 金额: $ 79.53万
• 依托单位: AMERICAN TYPE CULTURE COLLECTION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6256538
• 关键词: animal tissue; cell bank /registry; embryonic stem cell; genotype; molecular biology information system; sample collection; stem cells

Embryonal stem (ES) cells and lineage- or tissue-specific stem cells are important models in biomedical studies, but the availability and accessibility of research materials in this rapidly advancing field often become limiting. These restrictions can be addressed by a central, internationally recognized Stem Cell Resource devoted to acquisition and distribution of cells, reagents and information on nonhuman ES cells and postnatally derived stem cells from a variety of species. ATCC is the oldest, largest and most experienced organization engaging in this type of endeavor. Mechanisms for efficient and cost-effective accessioning, characterization, expansion, preservation and distribution of biomaterials and information are firmly in place and highly utilized daily. To a large degree these procedures were developed at ATCC out of necessity, providing an atmosphere fostering continued experimentation and improvement. In addition, several ATCC scientists are pursuing research relevant to stem cell biology, providing a critical research orientation to the Resource. We propose to accession, characterize, expand, preserve and distribute nonhuman ES cells, as well as postnatally derived stem cells from a variety of strains and species. Through a consortium arrangement under the direction of Dr. Barbara Knowles at The Jackson Laboratories, germline competency of murine ES cells will be confirmed in a quantitative manner prior to distribution, - We will also carry out Resource-related research, in partnership with Dr. James Thomson, Univ. of Wisconsin, to extensively characterize stem cells with regard to biological properties and utility of molecular tools used in stem cell research. We will standardize and opting methodology and develop new reagents in an attempt to make procedures and reagents broadly applicable across a range of species and strains. We will also establish interactive, web-based mechanisms to distribute information and technology as well as materials, and serve as a centralized source for information exchange and rapid dissemination of advances in this extremely active field.

胚胎干细胞和谱系或组织特异性干细胞是生物医学研究中的重要模型，但是在这个迅速前进的领域，研究材料的可用性和可访问性通常会受到限制。这些限制可以通过中央，国际认可的干细胞资源来解决，该干细胞资源致力于对非人类ES细胞的获取和分布，并从多种物种中获得非人类ES细胞以及产后衍生的干细胞。 ATCC是最古老，最大，最有经验的组织，参与此类努力。生物材料和信息的有效且具有成本效益的康复，表征，扩展，保存和分布的机制已牢固地适当，并每天都有高度使用。这些程序在很大程度上是出于必要性而在ATCC开发的，从而提供了一种促进持续的实验和改进的气氛。此外，一些ATCC科学家正在寻求与干细胞生物学相关的研究，从而为资源提供了重要的研究取向。我们建议康复，表征，扩展，保存和分布非人类ES细胞，以及来自多种菌株和物种的产后衍生的干细胞。通过在杰克逊实验室的芭芭拉·诺尔斯（Barbara Knowles）博士的指导下的联盟安排，将在分发之前以定量方式确认鼠类ES细胞的种系能力， - 我们还将与联合国大学的詹姆斯·托姆森（James Thomson）博士合作进行与资源相关的研究。威斯康星州的生物学特性以及干细胞研究中使用的分子工具的实用性，以广泛的特征。我们将标准化和选择方法，并开发新的试剂，以试图制造在各种物种和菌株中广泛适用的程序和试剂。 我们还将建立基于互动的，基于网络的机制，以分发信息和技术以及材料，并作为信息交换的集中式来源，并在这个极为活跃的领域中快速传播进步。