Non-Invasive Biomonitoring of Pesticides
农药的非侵入式生物监测
基本信息
- 批准号:7141457
- 负责人:
- 金额:$ 44.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-09-01 至 2011-08-31
- 项目状态:已结题
- 来源:
- 关键词:biomedical equipment developmentbiosensor devicecholinesterase inhibitorsclearance rategas chromatography mass spectrometrylaboratory ratmethod developmentminiature biomedical equipmentmodel design /developmentnoninvasive diagnosisoccupational hazardorganophosphorus insecticidepesticide biological effectpharmacokineticssaliva
项目摘要
DESCRIPTION (provided by applicant): This research project will establish a non-invasive biomonitoring capability to evaluate exposure to organophosphorus (OP) insecticides utilizing a sensitive, non-invasive, micro-analytical instrument for realtime analysis of biomarkers of exposure and response in saliva. This project will create a miniaturized nanobioelectronic biosensor that is highly selective and sensitive for the target analyte(s). In addition, a physiologically based pharmacokinetic and pharmacodynamic model (PBPK/PD) for the OP insecticide chlorpyrifos will be modified to incorporate a salivary gland compartment that will be used to quantitatively predict blood chlorpyrifos concentration and saliva cholinesterase (ChE) inhibition to estimate exposure based on a saliva specimen. The utilization of saliva for biomonitoring, coupled to real-time monitoring and modeling is a novel approach with broad application for evaluating occupational exposures to insecticides. An OP sensor will be developed based on a new biosensing principle of antigen-induced formation of nanoparticle-immuno complex nanostructure. A ChE sensor will also be developed based on the electrodetection of the ChE hydrolyzed reaction products choline and hydrogen peroxide. Subsequently, the sensors will be transformed to a "lab-on-a-chip", and the biochip performance will be characterized, optimized and validated. To validate this approach the pharmacokinetics of chlorpyrifos excretion and ChE inhibition in saliva under various physiological conditions and dose levels will be assessed to ensure that these endpoints are accurate predictors of internal dose. The development of a real-time saliva analysis coupled to a predictive pharmacokinetic model represents a significant advancement over current biomonitoring strategies. Once this model system has been adequately validated it can readily be employed to assess worker exposure to insecticides under a wide range of occupational situations.
描述(由申请人提供):该研究项目将建立一种非侵入性生物监测能力,利用灵敏、非侵入性微量分析仪器实时分析唾液中暴露和反应的生物标志物,评估有机磷(OP)杀虫剂的暴露情况。该项目将创建一种对目标分析物具有高度选择性和敏感性的小型化纳米生物电子生物传感器。此外,OP杀虫剂毒死蜱的基于生理学的药代动力学和药效学模型(PBPK/PD)将被修改,以纳入唾液腺室,该室将用于定量预测血液毒死蜱浓度和唾液胆碱酯酶(ChE)抑制,以估计基于暴露的暴露在唾液样本上。利用唾液进行生物监测,并结合实时监测和建模是一种具有广泛应用的评估杀虫剂职业接触的新方法。 OP传感器将基于抗原诱导形成纳米颗粒-免疫复合纳米结构的新生物传感原理而开发。还将基于对 ChE 水解反应产物胆碱和过氧化氢的电检测来开发 ChE 传感器。随后,传感器将转变为“芯片实验室”,生物芯片的性能将得到表征、优化和验证。为了验证该方法,将评估各种生理条件和剂量水平下毒死蜱排泄和唾液中 ChE 抑制的药代动力学,以确保这些终点是内部剂量的准确预测因子。实时唾液分析与预测药代动力学模型相结合的发展代表了当前生物监测策略的重大进步。一旦该模型系统得到充分验证,它就可以很容易地用于评估工人在各种职业情况下接触杀虫剂的情况。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CHARLES TIMCHALK其他文献
CHARLES TIMCHALK的其他文献
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{{ truncateString('CHARLES TIMCHALK', 18)}}的其他基金
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用于放射性核素修饰的选择性纳米孔吸附剂的开发
- 批准号:
7585451 - 财政年份:2006
- 资助金额:
$ 44.97万 - 项目类别:
Portable Analyzer for On-site Monitoring of Worker Exposure to Toxic Metals
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7282746 - 财政年份:2006
- 资助金额:
$ 44.97万 - 项目类别:
Development of Selective Nanoporous Sorbents for Radionuclide Decorporation
用于放射性核素修饰的选择性纳米孔吸附剂的开发
- 批准号:
7267893 - 财政年份:2006
- 资助金额:
$ 44.97万 - 项目类别:
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