Novel organic-inorganic hybrid nanoparticles for improving delivery of nucleic acid therapeutics in cancer therapy.

新型有机-无机杂化纳米粒子，用于改善癌症治疗中核酸疗法的传递。

• 批准号: 2744826
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2744826
• 关键词: Novel; organic; inorganic; hybrid; nanoparticles

This project aims to develop novel organic-inorganic hybrid nanomaterials for improving the delivery of nucleic acid therapeutics in cancer therapy. Specific objectives of the project include studying the synthesis and physicochemical characterisation of novel polyelectrolyte complex templated hybrid nanoparticles. The loading, release, and stability of nucleic acid therapeutics such as oligonucleotides and/or messenger RNA (mRNA) will be investigated using fluorescence spectroscopy and gel electrophoresis. Lastly, the biological safety, cellular uptake, and gene transfection efficacy of the nucleic acid loaded hybrid nanoparticles will be evaluated in 2D and 3D cell cultures.

该项目旨在开发新型有机-无机杂化纳米材料，以改善癌症治疗中核酸疗法的输送。该项目的具体目标包括研究新型聚电解质复合物模板混合纳米粒子的合成和物理化学表征。将使用荧光光谱和凝胶电泳研究寡核苷酸和/或信使 RNA (mRNA) 等核酸治疗剂的装载、释放和稳定性。最后，将在 2D 和 3D 细胞培养物中评估负载核酸的混合纳米颗粒的生物安全性、细胞摄取和基因转染功效。