Bacterial cell envelope remodelling in Rhizobium leguminosarum: contribution to symbiosis and resistance to abiotic stress

豆根瘤菌的细菌细胞包膜重塑：对共生和抵抗非生物胁迫的贡献

基本信息

批准号：
2744522
负责人：
金额：
--
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2744522
关键词：
Bacterial cell envelope remodelling Rhizobium

项目摘要

The aim of this project is to investigate how bacterial cellenvelope remodelling by Ldts contributes to symbiosis (inpea and bean) and to resistance to abiotic stress (heat,osmotic pressure, or acidic conditions). We will alsoinvestigate peptidoglycan structure and dynamics acrossrhizobium isolates to understand how cell envelopecomposition impacts persistence and fitness in the soil.This project will lay the foundation to engineer Rhizobiumstrains for sustainable agriculture.This multidisciplinary project involves 3 academicsupervisors with complementary expertise, based inSheffield (where most of the experimental work will becarried out), York and Oxford. The student will benefit froma training in biochemistry (HPLC and mass spectrometryfor peptidoglycan structural analysis, recombinant proteinexpression, enzymatic assays), bacterial genetics(construction of mutants), comparative genomics andrhizobium-plant interactions. The student will also spend 4months abroad working with the industrial partner (ProteinMetrics, California) to contribute to the development ofsoftware tools dedicated to "peptidoglycomics" analyses.Overall, the student will acquire a comprehensive set ofskills to explore mechanistic insights at molecular andcellular level in bacteria, and in the context of theinteraction with the plant.

该项目的目的是研究LDT的细菌细菌cellenvelope重塑如何促进共生（INPEA和BEAN）以及对非生物胁迫（热，渗透压或酸性条件）的抗性。 We will alsoinvestigate peptidoglycan structure and dynamics acrossrhizobium isolates to understand how cell envelopecomposition impacts persistence and fitness in the soil.This project will lay the foundation to engineer Rhizobiumstrains for sustainable agriculture.This multidisciplinary project involves 3 academicsupervisors with complementary expertise, based inSheffield (where most of the experimental work will becarried out), York and牛津。该学生将受益于生物化学培训（HPLC和质谱法，用于肽聚糖的结构分析，重组蛋白Xpression，酶试验），细菌遗传学（突变体的构造），比较基因组学且植物植物的相互作用。该学生还将与工业合作伙伴（加利福尼亚州的蛋白质伙伴）一起在国外花费4个月，以促进专门针对“ peptidogglycomics”分析的软件工具的开发。毕竟，学生将获得一个全面的技能，以探索在细菌和植物中的分子和细胞层面上的机械见解，以及该工厂的上下文。