Biosensor for real-time chemical monitoring

用于实时化学监测的生物传感器

• 批准号: 7224735
• 负责人: John R Cashman
• 金额: $ 58.58万
• 依托单位: HUMAN BIOMOLECULAR RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7224735
• 关键词: biomarker

DESCRIPTION (provided by applicant): Changes in the security situation facing citizens in the United States and military personnel abroad have greatly increased the threat that chemical weapons will be used against American forces and American civilians in the field as well as at home. In addition, hundreds of thousands of Americans are exposed to significant levels of pesticide organophosphates (OP) every year. These situations point to an urgent need for an efficient, fieldable and inexpensive way to detect OP. Detection instrumentation is an essential component of any protection paradigm, and thus a challenge is to develop sensitive and inexpensive detection methodology for various OP agents. The goal of our work is to be able to detect biomarkers of OP nerve agent exposure that can be readily and inexpensively deployed using a portable automated multianalyte biosensor. Our long-term goals are to develop a sensitive biosensor to detect biomarkers of OP exposure to provide information to protect populations at risk for exposure to chemical warfare agents or OP pesticides. The Aims for this research include: Aim 1) Design and synthesize biomarker reagents for a system to detect nerve agents including tabun, sarin, soman, VX, GF and tabun; Aim 2) Prepare and test antibodies to biomarkers of nerve agent exposure; Aim 3) Obtain biological samples from animals exposed to nerve agents or their surrogates; Aim 4) Develop the biosensor for nerve agent exposure and Aim 5) Build and test the final biosensor. Biochemical validation and development of a robust, portable Array Biosensor employing the optimized reagents and using currently available technology will be done in collaboration with the Naval Research Laboratory. With the success of the proposed work, we will obtain: 1) new reagents and technology for identifying OP-biomolecule conjugates of relevance to nerve agent and pesticide OP exposure, 2) verification of the robustness of the technology with biological samples, 3) development of an array-based methodology to selectively detect OP and 4) elaboration and testing of an Array Biosensor in the field with biological samples taken from animals exposed to low doses of nerve agents or nerve agent analogs. The final sensitive and selective product will be of use in a rapid screening format of physiological fluids for OP exposure.

描述（由申请人提供）：美国公民和海外军事人员面临的安全形势的变化大大增加了化学武器在战场上和国内对美国军队和美国平民使用的威胁。此外，每年有数十万美国人接触大量有机磷酸酯 (OP) 农药。这些情况表明迫切需要一种高效、可现场使用且廉价的方法来检测 OP。检测仪器是任何保护范例的重要组成部分，因此面临的挑战是为各种OP代理开发灵敏且廉价的检测方法。我们工作的目标是能够检测 OP 神经毒剂暴露的生物标志物，这些生物标志物可以使用便携式自动化多分析物生物传感器轻松且廉价地部署。我们的长期目标是开发一种灵敏的生物传感器来检测 OP 暴露的生物标志物，以提供信息来保护面临化学战剂或 OP 农药暴露风险的人群。本研究的目的包括： 目的 1) 设计和合成用于检测塔崩、沙林、索曼、VX、GF 和塔崩等神经毒剂的系统的生物标志物试剂；目标 2) 制备并测试神经毒剂暴露生物标志物的抗体；目标 3) 从暴露于神经毒剂或其替代物的动物中获取生物样本；目标 4) 开发用于神经毒剂暴露的生物传感器，目标 5) 构建并测试最终的生物传感器。将与海军研究实验室合作，利用优化的试剂和现有技术来进行生化验证和开发强大的便携式阵列生物传感器。随着拟议工作的成功，我们将获得：1）用于识别与神经毒剂和农药OP暴露相关的OP-生物分子缀合物的新试剂和技术，2）用生物样品验证该技术的稳健性，3）开发选择性检测 OP 的基于阵列的方法的研究和 4) 使用取自暴露于低剂量神经毒剂或神经毒剂类似物的动物的生物样本，在现场详细阐述和测试阵列生物传感器。最终的敏感和选择性产品将用于快速筛选生理液体中的 OP 暴露。