Biosensor for real-time chemical monitoring

用于实时化学监测的生物传感器

• 批准号: 7644836
• 负责人: John R Cashman
• 金额: $ 84.92万
• 依托单位: HUMAN BIOMOLECULAR RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7644836
• 关键词: Active Sites; Agriculture; American; Animals; Antibodies; Biochemical; Biochemistry; Biological; Biological Assay; Biological Markers; Biosensor; Blood; Buffers; Chemical Warfare; Chemical Warfare Agents; Chemical Weapons; Chemicals; Collaborations; Computer software; Detection; Development; Dose; Exposure to; Food Safety; Goals; Home environment; Human; Immunochemistry; Laboratory Research; Liquid substance; Medical; Methodology; Methods; Microfluidic Microchips; Military Personnel; Molecular; Molecular Biology; Monitor; Organophosphates; Peptides; Pesticides; Phase; Physiological; Populations at Risk; Reagent; Research; Sampling; Sarin; Screening procedure; Security; Site; Soman; Surface; Synthesis Chemistry; System; Technology; Testing; Time; Tissues; United States; Validation; Work; analog; base; brain tissue; design; detector; flexibility; improved; instrumentation; nerve agent; prototype; rapid detection; sensor; success; tabun; toxic organophosphate insecticide exposure

DESCRIPTION (provided by applicant): Changes in the security situation facing citizens in the United States and military personnel abroad have greatly increased the threat that chemical weapons will be used against American forces and American civilians in the field as well as at home. In addition, hundreds of thousands of Americans are exposed to significant levels of pesticide organophosphates (OP) every year. These situations point to an urgent need for an efficient, fieldable and inexpensive way to detect OP. Detection instrumentation is an essential component of any protection paradigm, and thus a challenge is to develop sensitive and inexpensive detection methodology for various OP agents. The goal of our work is to be able to detect biomarkers of OP nerve agent exposure that can be readily and inexpensively deployed using a portable automated multianalyte biosensor. Our long-term goals are to develop a sensitive biosensor to detect biomarkers of OP exposure to provide information to protect populations at risk for exposure to chemical warfare agents or OP pesticides. The Aims for this research include: Aim 1) Design and synthesize biomarker reagents for a system to detect nerve agents including tabun, sarin, soman, VX, GF and tabun; Aim 2) Prepare and test antibodies to biomarkers of nerve agent exposure; Aim 3) Obtain biological samples from animals exposed to nerve agents or their surrogates; Aim 4) Develop the biosensor for nerve agent exposure and Aim 5) Build and test the final biosensor. Biochemical validation and development of a robust, portable Array Biosensor employing the optimized reagents and using currently available technology will be done in collaboration with the Naval Research Laboratory. With the success of the proposed work, we will obtain: 1) new reagents and technology for identifying OP-biomolecule conjugates of relevance to nerve agent and pesticide OP exposure, 2) verification of the robustness of the technology with biological samples, 3) development of an array-based methodology to selectively detect OP and 4) elaboration and testing of an Array Biosensor in the field with biological samples taken from animals exposed to low doses of nerve agents or nerve agent analogs. The final sensitive and selective product will be of use in a rapid screening format of physiological fluids for OP exposure.

描述（由申请人提供）：美国和国外军事人员面临的安全状况的变化大大增加了将化学武器用于该领域和家里的美军和美国平民的威胁。此外，每年有成千上万的美国人暴露于大量的农药有机磷酸盐（OP）。这些情况表明迫切需要一种检测OP的高效，可实地和廉价的方式。检测仪器是任何保护范式的重要组成部分，因此挑战是为各种OP代理开发敏感且廉价的检测方法。我们工作的目的是能够检测可容易使用便携式自动化的多洋分析物生物传感器来容易且廉价地部署的OP神经毒剂暴露的生物标志物。我们的长期目标是开发一种灵敏的生物传感器，以检测OP暴露的生物标志物，以提供信息，以保护有可能接触化学战剂或OP农药的人群。这项研究的目的包括：目标1）设计和合成系统的生物标志物试剂，以检测包括Tabun，Sarin，Soman，VX，GF和Tabun等神经剂；目标2）准备和测试对神经毒剂暴露的生物标志物的抗体；目标3）从暴露于神经毒剂或其替代物的动物中获取生物样品；目标4）开发用于神经毒剂暴露的生物传感器并瞄准5）构建和测试最终的生物传感器。使用优化的试剂并使用当前可用技术的生化验证和开发，可与海军研究实验室合作进行。 With the success of the proposed work, we will obtain: 1) new reagents and technology for identifying OP-biomolecule conjugates of relevance to nerve agent and pesticide OP exposure, 2) verification of the robustness of the technology with biological samples, 3) development of an array-based methodology to selectively detect OP and 4) elaboration and testing of an Array Biosensor in the field with biological samples taken from animals exposed to low doses神经剂或神经剂类似物的象征。最终的敏感和选择性产品将以生理流体的快速筛选形式用于OP暴露。