INTEGRATING LCEC/LCM IN A SINGLE METABOLOMICS PLATFORM

将 LCEC/LCM 集成到单一代谢组学平台中

• 批准号: 7281832
• 负责人: WAYNE R MATSON
• 金额: $ 11.43万
• 依托单位: ESA BIOSCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7281832
• 关键词: NIH Roadmap Initiative tag; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biosensor device; biotechnology; clinical research; electrochemistry; high throughput technology; human tissue; mass spectrometry; metabolomics; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): Our broad objective is to extend and integrate electrochemical (EC) and MS technologies to facilitate a range of metabolomics studies. Our hypothesis is that hyphenated EC-MS will allow for greater elucidation of the metabolome is supported by our preliminary studies, which show that parallel EC-Array and MS substantially extends the scope, qualitative and quantitative capabilities of LC analysis. Additionally, serial EC can assist and control MS ionization and mimic biological redox reactions. Scaling of EC from synthetic to high through put and nanoscale formats and variants of electrode and electronic control allow optimization for specific applications. The specific aims are to: 1. Integrate existing EC and MS technologies for modular use in serial and parallel configurations adaptable to several LC-MS systems. This hyphenated approach for metabolomics will be evaluated with well-characterized multivariate methods to serve as a performance benchmark. 2. Develop innovative EC sensors and reactors, software and detection modalities to substantially extend the range, throughput, quantitative, and qualitative capabilities of both EC and MS as compared to the benchmark. 3. Characterize the developed EC and MS technologies and implement novel strategies for structural elucidation of metabolites and develop a shared knowledge base of qualitative information for use by our collaborators. 4. Characterize technology relevance to multivariate analysis, biological resolution and biomarker discovery using models of xenobiotic toxicity in rats and the widely used biological model C. Elegans. 5. Transfer technology to laboratories with active metabolomics programs to help establish specifications for formal development. 6. Implement production of the technology for wide dissemination to the metabolomics research community.

描述（由申请人提供）：我们的总体目标是扩展和集成电化学 (EC) 和 MS 技术，以促进一系列代谢组学研究。我们的假设是，联用 EC-MS 将能够更好地阐明代谢组，这一点得到我们的初步研究的支持，这些研究表明并行 EC 阵列和 MS 大大扩展了 LC 分析的范围、定性和定量能力。此外，串联 EC 可以辅助和控制 MS 电离并模拟生物氧化还原反应。 EC 从合成扩展到高通量和纳米级格式以及电极和电子控制的变体可以针对特定应用进行优化。具体目标是： 1. 集成现有的 EC 和 MS 技术，以便在适用于多种 LC-MS 系统的串行和并行配置中进行模块化使用。这种代谢组学的联用方法将使用特征良好的多变量方法进行评估，作为性能基准。 2. 开发创新的 EC 传感器和反应器、软件和检测方式，与基准相比，大幅扩展 EC 和 MS 的范围、通量、定量和定性能力。 3. 表征已开发的 EC 和 MS 技术，实施代谢物结构阐明的新策略，并开发定性信息的共享知识库供我们的合作者使用。 4. 使用大鼠外源性毒性模型和广泛使用的线虫生物模型来表征与多变量分析、生物分辨率和生物标志物发现相关的技术。 5. 将技术转让给具有活跃代谢组学项目的实验室，以帮助建立正式开发的规范。 6. 实施该技术的生产，以向代谢组学研究界广泛传播。