Sensor for Paralytic Shellfish Poisons

麻痹性贝类毒物传感器

• 批准号: 7157046
• 负责人: ROBERT E. GAWLEY
• 金额: $ 10万
• 依托单位: WHITE RIVER BIOSCIENCE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-08 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157046
• 关键词: algae; animal extract; chemical synthesis; covalent bond; fish poison; fluorescence; glass; marine toxins; paralysis; saxitoxin; seafood poisoning; surface coating; surface property; technology /technique development; water sampling /testing

DESCRIPTION (provided by applicant): Harmful Algal Blooms, or "HAB's", are linked to many cases of human poisoning each year. Economic losses and costs to the fishing industry, public health, and tourism are estimated to be $40,000,000 annually, as of the late 1990s. 1 such HAB causes paralytic shellfish poisoning (PSP). Following an algal bloom, the toxins released by the microbes may accumulate in filter-feeding shellfish, consumption of which then causes disease symptoms, the most severe of which is respiratory paralysis and death. PSPs are 1 of only a few marine toxins that can be fatal to humans when consumed in food. This proposal seeks funding to develop recently reported technology for the fluorescence detection of PSPs into fluorescent detector plates. In Phase I, the limits of detection of 4 PSPs will be determined, and a preliminary evaluation of fluorescence response in toxic shellfish extracts will be conducted. Such plates will be adapted for use in a portable device in Phase II. The Specific Aims of this project are: 1. Synthesize fluorescent chemosensors suitable for covalent attachment to a glass slide. 2. Self-assembly and covalent attachment of the sensors to glass slides and evaluation of the surface properties and coating. 3. Evaluation of the fluorescence amplification to solutions of saxitoxin, PSP toxins, and toxic shellfish extracts. Paralytic Shellfish Poisons (PSPs) enter the human food chain most commonly through filter-feeding shellfish transvectors. The most toxic PSP, saxitoxin, is also 1 of only 3 "small molecules" on the government's list of "Select Agents". Fast, inexpensive means of detection PSPs is desirable.

描述（由申请人提供）：有害的藻华或“ HAB”与每年的许多人中毒案有关。截至1990年代后期，捕鱼行业，公共卫生和旅游业的经济损失和成本估计为40,000,000美元。 1这种HAB引起麻痹贝类中毒（PSP）。在藻类开花之后，微生物释放的毒素可能会积聚在过滤器喂养的贝类中，然后消耗会引起疾病症状，其中最严重的是呼吸瘫痪和死亡。 PSP是只有几种海洋毒素中的1个，在食物中食用时可能对人类致命。该提案寻求资金开发最近报告的技术，用于将PSP荧光检测到荧光探测器板中。在第一阶段，将确定4个PSP的检测限制，并将对有毒贝类提取物中的荧光反应进行初步评估。此类板将适用于在II阶段的便携式设备中使用。该项目的具体目的是：1。合成荧光化学传感器，适合于载玻片的共价附件。 2。传感器对载玻片的自组装和共价附着以及对表面特性和涂层的评估。 3。评估荧光扩增对萨克西毒素，PSP毒素和有毒贝类提取物的溶液的评估。瘫痪的贝类毒物（PSP）通常是通过过滤式贝类转向向量进入人类食物链。最有毒的PSP Saxitoxin也是政府“精选代理”名单上仅3个“小分子”中的1个。需要快速，廉价的检测手段PSP。