CRYOPRESERVATION OF SEMEN AND SOMATIC CELLS

精液和体细胞的冷冻保存

• 批准号: 7391947
• 负责人: MARK E HASKINS
• 金额: $ 0.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391947
• 关键词: CRYOPRESERVATION; SEMEN; SOMATIC; CELLS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Semen from carriers or affected dogs affected with genetic diseases that are known or suspected animal models of human genetic disease is collected, diluted, frozen, and stored for the future use. Somatic cells, usually fibroblasts, are grown in culture, harvested, and frozen in liquid nitrogen for later re-culture and study by a number of methods including enzyme assays and DNA Southern blotting/pcr amplification of specified markers for linkage studies, as well as cytogenetic studies. Feline semen samples are collected, frozen, and stored by Drs. JoGayle Howard and Budda Pukazheathi, Genome Resources Bank, Smithsonian Institution, National Zoological Park, Washington DC. Frozen feline embryos are made and stored by Dr. William Swanson, Cincinnati Zoo, in collaboration with his NCRR grant. We have also provided testes from dogs and cats to Drs. Ralph Brinster and Ina Dobrinski for cross-species germ cell transplantation studies. We store semen and fibroblasts from goats with beta-mannosidosis and mucopolysaccharidosis IIID provided by Dr. Margaret Jones, Michigan State University. These colonies were threatened by Dr. Jones retirement, and the semen and fibroblast resources are being stored in liquid nitrogen.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。收集，稀释，冷冻和储存的人类遗传疾病动物模型的载体或受影响的狗的精液供未来使用。体细胞（通常是成纤维细胞）在培养物中生长，收获和冷冻在液氮中，以便以后再培养，并通过多种方法进行研究，包括酶测定和DNA Southern印迹/PCR扩增指定的标记，以进行链接研究，以及细胞遗传学研究。 收集，冷冻和由DRS存储的猫精液样品。 Jogayle Howard和Budda Pukazheathi，Genome Resources Bank，Smithsonian Institution，National Zoological Park，Washington DC。冷冻的猫胚是由辛辛那提动物园的威廉·斯旺森博士与他的NCRR Grant合作制作和存储的。我们还向Drs提供了狗和猫的睾丸。 Ralph Brinster和Ina Dobrinski用于跨物种生殖细胞移植研究。我们存储来自密歇根州立大学玛格丽特·琼斯（Margaret Jones）博士提供的β-甘露糖性和粘膜糖含量IIID的山羊的精液和成纤维细胞。这些殖民地受到琼斯退休博士的威胁，精液和成纤维细胞资源正在液氮中存储。