Molecular Biology of Alcohol Metabolism

酒精代谢的分子生物学

• 批准号: 6882378
• 负责人: HELEN Lindsay THOMPSON
• 金额: $ 10万
• 依托单位: EDVOTEK, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6882378
• 关键词: alcohol dehydrogenase; alcoholic beverage consumption; alcoholism /alcohol abuse education; clinical research; educational resource design /development; ethanol; genetic polymorphism; genetic techniques; hair follicle; human genetic material tag; metabolism; polymerase chain reaction; restriction fragment length polymorphism; technology /technique development

DESCRIPTION (provided by applicant): Alcohol consumption is a worldwide public health issue of great significance and studies indicate that adolescent alcohol misuse is on the increase. Research has also shown that variations in alcohol metabolizing enzymes controlled by genetic polymorphisms influence the metabolism of alcohol, and thereby significantly affect drinking behavior, the development of alcoholism and alcohol-induced organ damage. In this proposal, Edvotek, Inc. will investigate the feasibility of inquiry-based health educational resources focused on genetic factors that control the metabolism of alcohol with the hope that such experiments and related-material might support student decision-making processes in choosing not to drink and/or decrease the amount consumed. The proposed educational products will build on molecular biology concepts and equipment currently available in high schools. The polymerase chain reaction (PCR) will be used to examine polymorphism's in the alcohol dehydrogenase (ADH3) gene that correlates with altered alcohol metabolism. In Phase I, we will research an educational PCR-based system coupled with restriction fragment length polymorphism analysis (RFLP) for the detection of two ADH3 alleles that produce enzymes with altered alcohol metabolism. We will also develop a simple and inexpensive method to isolate DNA from hair follicles. This phase I proposal will implement strategies to protect the confidentiality of human subjects, provisions for inclusion of children, women and minorities, and a debriefing component for classroom use. While the educational resources developed in this proposal will complement existing classroom experiments in some schools, we will also explore the feasibility of using a leaner program, designated as the BIOREEP program, to provide all the needed molecular biology equipment to schools which lack such resources. Additionally, we propose strategies for connecting these products to the National Science Education Standards. The impacts of the proposed project are 1) sensitivity to issues of alcohol metabolism as it relates to alcohol consumption; and 2) an appreciation in understanding genetic background as it relates to alcohol abuse and other diseases.

描述（由申请人提供）：饮酒是一个具有重要意义的全球公共卫生问题，研究表明，青少年滥用酗酒正在增加。研究还表明，遗传多态性控制的酒精代谢酶的变化会影响酒精的代谢，从而显着影响饮酒行为，酒精中毒和酒精诱导的器官损害的发展。在该提案中，Edvotek，Inc。将调查基于询问的健康教育资源的可行性，这些健康教育资源的可行性集中在控制酒精的代谢的遗传因素上，希望这种实验和相关物质可以支持学生决策过程，以选择不喝酒和减少消耗的量。拟议的教育产品将基于当前高中目前可用的分子生物学概念和设备。聚合酶链反应（PCR）将用于检查与酒精代谢改变相关的酒精脱氢酶（ADH3）基因中的多态性。在第一阶段，我们将研究一个基于教育PCR的系统，以及限制片段长度多态性分析（RFLP），用于检测两个ADH3等位基因，这些ADH3等位基因产生了含酒精代谢改变的酶。我们还将开发一种简单且廉价的方法来分离毛囊的DNA。该阶段I提案将实施保护人类受试者机密性，包括儿童，妇女和少数民族的规定以及课堂使用的汇报组成部分的战略。尽管本提案中开发的教育资源将补充某些学校的现有课堂实验，但我们还将探索使用被指定为生物EP计划的精益计划的可行性，以向缺乏此类资源的学校提供​​所有所需的分子生物学设备。此外，我们提出了将这些产品连接到国家科学教育标准的策略。拟议项目的影响是1）对酒精代谢问题的敏感性与饮酒有关； 2）了解与酒精滥用和其他疾病有关的遗传背景的欣赏。