Molecular Biology of Alcohol Metabolism

酒精代谢的分子生物学

• 批准号: 6882378
• 负责人: HELEN Lindsay THOMPSON
• 金额: $ 10万
• 依托单位: EDVOTEK, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6882378
• 关键词: alcohol dehydrogenase; alcoholic beverage consumption; alcoholism /alcohol abuse education; clinical research; educational resource design /development; ethanol; genetic polymorphism; genetic techniques; hair follicle; human genetic material tag; metabolism; polymerase chain reaction; restriction fragment length polymorphism; technology /technique development

DESCRIPTION (provided by applicant): Alcohol consumption is a worldwide public health issue of great significance and studies indicate that adolescent alcohol misuse is on the increase. Research has also shown that variations in alcohol metabolizing enzymes controlled by genetic polymorphisms influence the metabolism of alcohol, and thereby significantly affect drinking behavior, the development of alcoholism and alcohol-induced organ damage. In this proposal, Edvotek, Inc. will investigate the feasibility of inquiry-based health educational resources focused on genetic factors that control the metabolism of alcohol with the hope that such experiments and related-material might support student decision-making processes in choosing not to drink and/or decrease the amount consumed. The proposed educational products will build on molecular biology concepts and equipment currently available in high schools. The polymerase chain reaction (PCR) will be used to examine polymorphism's in the alcohol dehydrogenase (ADH3) gene that correlates with altered alcohol metabolism. In Phase I, we will research an educational PCR-based system coupled with restriction fragment length polymorphism analysis (RFLP) for the detection of two ADH3 alleles that produce enzymes with altered alcohol metabolism. We will also develop a simple and inexpensive method to isolate DNA from hair follicles. This phase I proposal will implement strategies to protect the confidentiality of human subjects, provisions for inclusion of children, women and minorities, and a debriefing component for classroom use. While the educational resources developed in this proposal will complement existing classroom experiments in some schools, we will also explore the feasibility of using a leaner program, designated as the BIOREEP program, to provide all the needed molecular biology equipment to schools which lack such resources. Additionally, we propose strategies for connecting these products to the National Science Education Standards. The impacts of the proposed project are 1) sensitivity to issues of alcohol metabolism as it relates to alcohol consumption; and 2) an appreciation in understanding genetic background as it relates to alcohol abuse and other diseases.

描述（由申请人提供）：酒精消费是一个具有重要意义的世界性公共卫生问题，研究表明青少年酗酒现象正在增加。研究还表明，由遗传多态性控制的酒精代谢酶的变异会影响酒精的代谢，从而显着影响饮酒行为、酒精中毒的发展和酒精引起的器官损伤。在这项提案中，Edvotek, Inc. 将调查基于探究的健康教育资源的可行性，重点关注控制酒精代谢的遗传因素，希望此类实验和相关材料可以支持学生选择不饮酒的决策过程。饮酒和/或减少消耗量。拟议的教育产品将建立在高中现有的分子生物学概念和设备的基础上。聚合酶链式反应 (PCR) 将用于检查与酒精代谢改变相关的乙醇脱氢酶 (ADH3) 基因的多态性。在第一阶段，我们将研究一个基于 PCR 的教育系统，结合限制性片段长度多态性分析 (RFLP)，用于检测两个 ADH3 等位基因，这些基因产生的酶会改变酒精代谢。我们还将开发一种简单且廉价的方法来从毛囊中分离 DNA。第一阶段提案将实施保护人类受试者机密性的策略、纳入儿童、妇女和少数群体的规定以及供课堂使用的汇报部分。虽然本提案中开发的教育资源将补充一些学校现有的课堂实验，但我们还将探讨使用更精简的计划（指定为 BIOREEP 计划）的可行性，为缺乏此类资源的学校提供​​所有所需的分子生物学设备。此外，我们还提出了将这些产品与国家科学教育标准联系起来的策略。拟议项目的影响是 1) 对酒精代谢问题的敏感性，因为它与饮酒有关； 2）了解与酗酒和其他疾病相关的遗传背景。