Development of rapid detection tests for B pseudomallei

假鼻疽杆菌快速检测方法的开发

• 批准号: 6994570
• 负责人: Raymond Houghton
• 金额: $ 46.57万
• 依托单位: INBIOS INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994570
• 关键词: Burkholderia; SDS polyacrylamide gel electrophoresis; bacterial antigens; bioinformatics; bioterrorism /chemical warfare; clinical research; diagnosis design /evaluation; diagnosis quality /standard; early diagnosis; enzyme linked immunosorbent assay; human tissue; immune complex; immunologic assay /test; laboratory mouse; microorganism classification; monoclonal antibody; rapid diagnosis; serotyping; systematic biology; western blottings

DESCRIPTION (provided by applicant): The Gram negative bacterium Burkholderia pseudomallei causes human melioidosis, a rapidly progressing and frequently fatal disease if not treated early. It is endemic in areas of NE Thailand, Southeast Asia and northern parts of Australia and is listed as a Category B agent of potential bioterrorist threats. Antibiotic treatment is difficult and prolonged with significant relapse rates and there is currently no vaccine available for protection of naturally or intentionally exposed individuals. Accurate tests for the rapid detection of this organism in blood samples and other clinical materials are needed to guide the critical early phases of clinical management but are not currently available. It is the purpose of this application to identify antigens specific to B. pseudomallei and use these to generate monoclonal antibodies for inclusion in a rapid field test for antigen detection particularly in acute infections. In addition, these antigens can be used directly to develop specific immunoblots for evaluating antibody responses in acute and chronic phases of disease. To achieve these goals, InBios (experienced in commercial development of rapid diagnostic assays) has brought together a team of experts in the UK with access to an animal model system for melioidosis with a group in Northeastern Thailand with access to clinical samples enabling Phase 1 field testing of the prototype assays produced. These tools will be invaluable in detecting and monitoring acute and chronic B. pseudomallei infections both in endemic areas as well as following a bioterrorist attack.

描述（由申请人提供）：革兰氏阴性细菌类鼻疽伯克霍尔德氏菌会引起人类类鼻疽，这是一种进展迅速且如果不及早治疗通常会致命的疾病。它在泰国东北部、东南亚和澳大利亚北部地区流行，被列为潜在生物恐怖威胁的 B 类病原体。抗生素治疗困难且耗时长，复发率很高，而且目前没有疫苗可用于保护自然或有意暴露的个体。需要进行准确的测试来快速检测血液样本和其他临床材料中的这种微生物，以指导临床管理的关键早期阶段，但目前尚不可用。本申请的目的是鉴定类鼻疽伯克氏菌特异性抗原，并使用这些抗原生成单克隆抗体，用于快速现场检测抗原检测，特别是在急性感染中。此外，这些抗原可直接用于开发特异性免疫印迹，以评估疾病急性期和慢性期的抗体反应。为了实现这些目标，InBios（在快速诊断分析的商业开发方面经验丰富）汇集了英国的专家团队和泰国东北部的一组专家，他们可以使用类鼻疽动物模型系统，而泰国东北部的团队可以使用临床样本，从而实现第一阶段的领域对所产生的原型测定进行测试。这些工具对于检测和监测流行地区以及生物恐怖袭击后的急性和慢性假鼻疽杆菌感染将具有无价的价值。