Beckman XL-I Analytical Ultracentrifuge

Beckman XL-I 分析超速离心机

• 批准号: 6733291
• 负责人: KRISHNA KUMAR
• 金额: $ 32.72万
• 依托单位: TUFTS UNIVERSITY MEDFORD
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733291
• 关键词: analytical ultracentrifugation; biomedical equipment purchase

DESCRIPTION (provided by applicant): This is a proposal to acquire a state-of-the-art Beckman Optima(TM) XL-I analytical ultracentrifuge equipped with both absorbance and Rayleigh interference optics. There are currently no analytical ultracentrifuges available at Tufts University on any of the three campuses. The investigator group is composed of seven users, six of whom are funded by the NIH and will serve as the core user group. Five of the seven investigators are from four different departments at Tufts A & S, and Engineering (Medford) and Tufts University School of Medicine (Boston). Two of the investigators are in the Department of Neurology at Harvard Medical School. The Director of the Analytical Ultracentrifugation Research Laboratory at the Boston Biomedical Research Institute will serve as an external advisor to the facility. In order to accomplish the aims of the NIH-funded research programs at Tufts and Harvard Medical School, the acquisition of an analytical ultracentrifuge has become essential. The need of these researchers for an analytical ultracentrifuge cannot be met under prevailing conditions. The user group identified here brings a broad spectrum of research backgrounds and expertise and is actively engaged in quantitatively probing macromolecular structure and function. Both equilibrium and velocity sedimentation will be used to determine thermodynamic and hydrodynamic parameters of molecular systems. The XL-I will greatly enhance the ability of the major users to determine descriptors associated with macromolecular interactions such as equilibrium constants, stoichiometries and molecular shapes. The analytical ultracentrifuge is uniquely suited to measure properties of macromolecules in solution as a function of pH, ionic strength and solution components. This instrument will fulfill an acute need of NIH supported investigators at Tufts and Harvard Medical School and greatly enrich the existing research infrastructure at Tufts.

描述（由申请人提供）： 这是一项购买配备吸光度和瑞利干涉光学器件的最先进的 Beckman Optima™ XL-I 分析超速离心机的提案。目前塔夫茨大学的三个校区均没有分析超速离心机。研究小组由七名用户组成，其中六名由 NIH 资助，将作为核心用户组。七名研究人员中的五名来自塔夫茨大学工程学院（梅德福）和塔夫茨大学医学院（波士顿）的四个不同部门。其中两名研究人员来自哈佛医学院神经病学系。波士顿生物医学研究所分析超速离心研究实验室主任将担任该设施的外部顾问。为了实现 NIH 资助的塔夫茨大学和哈佛医学院研究项目的目标，购买分析超速离心机变得至关重要。在普遍条件下无法满足这些研究人员对分析超速离心机的需求。这里确定的用户群体拥有广泛的研究背景和专业知识，并积极参与定量探索大分子结构和功能。平衡和速度沉降都将用于确定分子系统的热力学和流体动力学参数。 XL-I 将极大地增强主要用户确定与大分子相互作用相关的描述符的能力，例如平衡常数、化学计量和分子形状。分析超速离心机特别适合测量溶液中大分子的特性（作为 pH、离子强度和溶液成分的函数）。该仪器将满足 NIH 支持的塔夫茨大学和哈佛医学院研究人员的迫切需求，并极大地丰富塔夫茨大学现有的研究基础设施。

项目成果

A synthetic trivalent hapten that aggregates anti-2,4-DNP IgG into bicyclic trimers.

一种合成三价半抗原，可将抗 2,4-DNP IgG 聚合成双环三聚体。

• 发表时间: 2007-03-28
• 影响因子: 15
• 作者: Bilgiçer, Basar;Moustakas, Demetri T;Whitesides, George M
• 通讯作者: Whitesides, George M

Dependence of avidity on linker length for a bivalent ligand-bivalent receptor model system.

二价配体-二价受体模型系统的亲和力对接头长度的依赖性。

• DOI: 10.1021/ja2073033
• 发表时间: 2012-01-11
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Mack ET;Snyder PW;Perez-Castillejos R;Bilgiçer B;Moustakas DT;Butte MJ;Whitesides GM
• 通讯作者: Whitesides GM