Analysis of novel non coding mRNA-like RNAs in mice

小鼠中新型非编码 mRNA 样 RNA 的分析

• 批准号: 7272762
• 负责人: Antony Rodriguez
• 金额: $ 2.25万
• 依托单位: SANGER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7272762
• 关键词: RNA; bioinformatics; computational biology; gene targeting; genetically modified animals; laboratory mouse; molecular genetics; postdoctoral investigator

DESCRIPTION (provided by applicant): Large numbers of novel non-protein-coding RNAs (ncRNAs) have been characterized in worms, plants, insects, and mammals by experimental approaches. However, with the advent of large-scale genome sequencing, especially in humans and mice, literally hundreds of additional ncRNAs of unknown classification and function have been identified. In mice alone, the ncRNAs are thought to represent approximately 1/3 of all transcription units in the genome. ncRNA function remains poorly understood since past research has focused primarily on protein coding genes and the proteome. To begin to close the knowledge gap on these types of transcription units, firstly, bioinformatics-based filtering of mouse putative non coding mRNA-like RNAs identified by RIKEN-FANTOM will be conducted to identify novel candidates for functional studies Secondly, molecular expression analysis of a selected set of ncRNAs will be conducted to identify tissue specific and/or abundantly expressed transcripts. Following from the molecular analysis, a select group of non-coding RNAs will be analyzed by loss-of-function genetics in mice.

描述（由申请人提供）： 通过实验方法对蠕虫、植物、昆虫和哺乳动物中大量新型非蛋白编码 RNA (ncRNA) 进行了表征。然而，随着大规模基因组测序（尤其是人类和小鼠基因组测序）的出现，实际上已经鉴定出了数百种分类和功能未知的额外 ncRNA。仅在小鼠中，ncRNA 就被认为代表了基因组中所有转录单位的大约 1/3。 由于过去的研究主要集中在蛋白质编码基因和蛋白质组上，因此人们对 ncRNA 的功能仍然知之甚少。为了开始缩小这些类型转录单位的知识差距，首先，将基于生物信息学对 RIKEN-FANTOM 鉴定的小鼠推定非编码 mRNA 样 RNA 进行过滤，以确定用于功能研究的新候选者。将进行一组选定的 ncRNA 来鉴定组织特异性和/或大量表达的转录本。分子分析之后，将通过小鼠功能丧失遗传学对一组选定的非编码 RNA 进行分析。