Spine Dynamics Underlying Ocular Dominance Plasticity

眼部优势可塑性背后的脊柱动力学

基本信息

批准号：
7047748
负责人：
SPENCER LAVERE SMITH
金额：
$ 3.62万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2006-12-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): In the developing nervous system, initially promiscuous synaptic connections are remodeled by activity-dependent mechanisms, with axons strengthening their connections with some targets while completely disconnecting from others. In the neocortex, this process has been most thoroughly studied in the developing primary visual cortex, where decorrelating the activity of the two eyes dramatically reduces cortical binocularity. These functional changes in cortical physiology are widely accepted to be anchored in long-lasting changes in the efficacy of synaptic connections. However, the specific mechanisms by which synaptic plasticities lead to changes in the function of cortical circuitry remain elusive. The major objective of the work proposed here is to determine where in the cortical circuit experience-dependent changes in binocular responses are first observed, track the progression over time, and test the hypothesis that these changes are anchored in anatomical changes in synaptic connectivity. To address these questions, techniques such as 2-photon laser scanning microscopy, intrinsic signal optical imaging, and single-unit recordings, will be used to map circuit plasticity and follow changes in synaptic connectivity in vivo.

描述（由申请人提供）：在发育中的神经系统中，最初是通过活动依赖性机制重塑了混杂的突触连接，轴突可以加强与某些目标的连接，同时与其他目标完全断开。在新皮层中，该过程在发育中的主要视觉皮层中进行了最彻底的研究，在该皮层中，两只眼睛的活性显着降低了皮质双向性。皮质生理学中的这些功能变化被广泛接受为锚定在突触连接功效的持久变化中。但是，突触可塑性导致皮质回路功能变化的特定机制仍然难以捉摸。这里提出的工作的主要目的是确定首先观察到皮质回路中双眼响应的依赖性变化，跟踪随着时间的流逝的进展，并测试这些变化是在突触连接性的解剖学变化中锚定的假设。为了解决这些问题，将使用诸如2光子激光扫描显微镜，固有信号光学成像和单单元记录等技术来绘制电路可塑性并遵循体内突触连通性的变化。