Nonstationary Isotopic Tracer Analysis of Hepatocytes

肝细胞的非稳态同位素示踪分析

• 批准号: 7159345
• 负责人: Jamey D. Young
• 金额: $ 4.45万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7159345
• 关键词: analytical method; computer program /software; computer system design /evaluation; diabetes mellitus; laboratory mouse; liver cells; liver metabolism; postdoctoral investigator; stable isotope; technology /technique development

DESCRIPTION (provided by applicant): There are three specific aims of the project, which are to (a) develop a methodology for metabolic flux estimation based on nonstationary isotopic tracer experiments, (b) create a general-purpose software application for analysis and interpretation of nonstationary isotopic tracer experiments, and (c) to apply the aforementioned theoretical results and software tools to analyze hepatocyte metabolism under diabetic and normal conditions. This research will provide a deeper understanding of the mechanisms by which the liver responds to diabetic disease states, thus leading to novel strategies for disease treatment, monitoring, and diagnosis. By allowing tracer studies to be performed during the transient period preceding isotopic steadystate, the techniques developed in this project will permit flux estimates to be obtained within a shorter timeframe than traditional methods. Because the liver-specific functions of hepatocytes often degrade over time, it is important to analyze these cells soon after they are isolated from the liver to get an accurate picture of the in vivo flux state.

DESCRIPTION (provided by applicant): There are three specific aims of the project, which are to (a) develop a methodology for metabolic flux estimation based on nonstationary isotopic tracer experiments, (b) create a general-purpose software application for analysis and interpretation of nonstationary isotopic tracer experiments, and (c) to apply the aforementioned theoretical results and software tools to analyze hepatocyte metabolism在糖尿病和正常情况下。这项研究将对肝脏对糖尿病疾病状态反应的机制有更深入的了解，从而导致新的疾病治疗，监测和诊断策略。通过允许在同位素稳态之前的瞬态期间进行示踪剂研究，该项目中开发的技术将允许在较短的时间范围内获得与传统方法相比，在较短的时间范围内获得通量估计。由于肝细胞的肝特异性功能通常会随着时间的流逝而降解，因此在这些细胞与肝脏分离后不久分析它们以获取体内滤光片状态的准确图片很重要。