Anticancer Potential of Black Cohosh on Breast Cancer

黑升麻对乳腺癌的抗癌潜力

• 批准号: 7011261
• 负责人: LINDA S EINBOND
• 金额: $ 10.01万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7011261
• 关键词: alternative medicine; angiosperms; antineoplastics; apoptosis; athymic mouse; breast neoplasms; cell cycle; cell growth regulation; cell line; drug screening /evaluation; medicinal plants; pharmacokinetics; plant extracts; viral carcinogenesis; western blottings

DESCRIPTION (provided by applicant): My intent is to combine my background in microbiology and viral oncology with newer interests in cancer and herbal medicines. My objective is to initiate a productive career in research and teaching at a university or research institute that will lead to the development and clinical use of novel anticancer therapies and chemopreventive agents. The focus of this project will be mechanistic studies of herbal medicines, in particular black cohosh, on cancer in vitro and in vivo. The ideal mentor to accomplish this is Dr. I. Bernard Weinstein, who is an expert on molecular aspects of carcinogenesis and the ideal environment is Columbia University College of Physicians and Surgeons. Black cohosh, Actaea racemosa L. (= Cimicifuga racemosa), is a North American perennial in the buttercup family (Ranunculaceae) used for various women's health conditions. The overall objective of this project is to determine whether black cohosh contains components that may eventually be useful in the prevention or treatment of breast cancer. Indeed, our preliminary experiments indicate that black cohosh extracts inhibit the growth of human breast cancer cells. We will determine the pharmacokinetics of black cohosh. Partially purified extracts and specific chemicals will be examined for their effects, alone and in combination with chemotherapy agents, on the function of specific molecules that control cell growth, the cell cycle, apoptosis and the function of the ER and Her-2 receptor. Western blot analysis will be performed to examine possible effects on the expression of cyclin D1 related proteins. Luciferase reporter assays will be done to monitor effects on the transcription of NF-kB, fos, myc, jun and PPAR-g. Analytic chemical techniques will be used to identify novel compounds that might mediate these effects. To ascertain the efficacy of the compounds to treat humans, we will examine effects of an extract of black cohosh on xenografts of human breast cancer cell lines in athymic mice. This research project could identify new approaches to breast cancer prevention and therapy.

描述（由申请人提供）：我的目的是将我在微生物学和病毒肿瘤学方面的背景与对癌症和草药的新兴趣结合起来。我的目标是在大学或研究机构开展富有成效的研究和教学职业，这将导致新型抗癌疗法和化学预防药物的开发和临床应用。该项目的重点是草药（特别是黑升麻）对体外和体内癌症的作用机制研究。实现这一目标的理想导师是 I. Bernard Weinstein 博士，他是致癌分子方面的专家，而理想的环境是哥伦比亚大学内科和外科医生学院。黑升麻，Actaearacemosa L.（= Cimicifugaracemosa），是北美毛茛科（毛茛科）多年生植物，用于治疗各种女性健康状况。该项目的总体目标是确定黑升麻是否含有最终可用于预防或治疗乳腺癌的成分。事实上，我们的初步实验表明黑升麻提取物可抑制人类乳腺癌细胞的生长。我们将确定黑升麻的药代动力学。将检查部分纯化的提取物和特定化学物质单独或与化疗药物组合对控制细胞生长、细胞周期、细胞凋亡以及 ER 和 Her-2 受体功能的特定分子功能的影响。将进行蛋白质印迹分析以检查对细胞周期蛋白 D1 相关蛋白表达的可能影响。将进行荧光素酶报告基因检测以监测对 NF-kB、fos、myc、jun 和 PPAR-g 转录的影响。分析化学技术将用于识别可能介导这些效应的新型化合物。为了确定这些化合物对人类的疗效，我们将研究黑升麻提取物对无胸腺小鼠乳腺癌细胞系异种移植物的影响。该研究项目可以确定乳腺癌预防和治疗的新方法。