Develop a novel in vitro 3D bioelectric system of the GI with enteric nervous system using electronic scaffold devices

使用电子支架装置开发具有肠神经系统的新型胃肠道体外 3D 生物电系统

• 批准号: 2720560
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2720560
• 关键词: Develop; novel; vitro; 3D; bioelectric

This research project aims to produce a novel in vitro 3D bioelectric system of the GI tract with enteric nervous model of the human gastrointestinal track (GI) using electronic/photonic scaffold devices. Thus, allowing for more accurate model than those currently available using animal models/rodent cells. This will allow for continuous electrical monitoring of the bioelectric system, to allow for the observation of the effect of drugs on the electrical nature of GI/ENS over time for enteric pain and monitoring, as well as stimulation of the model through photo -stimulation. This project will mainly involve practical lab-based work growing the different cell cultures and further develop the hydrogel scaffolding to more accurately represent the human GI tract. Experimental techniques will include Electrochemical impedance spectroscopy (EIS), photostimulation, Transepithelial/transendothelial electrical resistance (TEER) and (confocal microscopy, SEM).

该研究项目旨在通过使用电子/光子支架设备的人类胃肠道轨迹（GI）的肠神经模型生产出一种新型的胃肠道体外3D生物电系统。因此，比当前使用动物模型/啮齿动物细胞可用的模型更准确。这将允许对生物电系统进行连续的电气监测，从而可以观察药物对肠内痛苦和监测的GI/ENS的影响，以及通过照片刺激来刺激模型。该项目将主要涉及基于实验室的实际工作，种植不同的细胞培养物，并进一步发展水凝胶脚手架，以更准确地代表人类胃肠道。实验技术将包括电化学阻抗光谱（EIS），光刺激，跨皮/跨内皮电阻（TEER）和（共聚焦显微镜，SEM）。