Neural Probes for Electrical and Chemical Sensing

用于电气和化学传感的神经探针

• 批准号: 7140662
• 负责人: DARYL R KIPKE
• 金额: $ 17.6万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7140662
• 关键词: acetylcholine; bioengineering /biomedical engineering; biosensor device; dopamine; drug delivery systems; electrochemistry; electrophysiology; enzyme electrodes; fluorescent dye /probe; glutamates; implant; laboratory rat; medical implant science; microfluidics; neurochemistry; neuropharmacology; polymers; serotonin; silicon; technology /technique development

DESCRIPTION (provided by applicant): Our long-term research goal is to develop implantable neurotechnologies for long-term and high fidelity microscale electrical and chemical interfaces to the central nervous system. The objective of this two-year project is to develop a new type of multi-modal neural probe by integrating chemical sensors on microfabricated thin-film silicon devices that are presently used for neural recording, stimulation, and microscale drug injection. The first specific aim is to develop and characterize integrated chemical sensing of two electroactive neurochemicals (dopamine and serotonin), along with concurrent electrophysiological recording. The primary tasks include developing electrode site materials, integrating a reference electrode onto the probe substrate, and characterizing 'electrode site spacing for concurrent neurochemical sensing and electrophysiological recording. The second specific aim is to develop arid characterize integrated chemical sensing of two non-electroactive neurochemicals (acetylcholine and glutamate), along with concurrent electrophysiological recording and microfluidic delivery of pharmacological agents. The primary tasks of this aim include developing polymer wells and conductive polymer coatings for enzyme entrapment and characterizing sensor performance. In both aims, the multi-modal devices will be quantitatively evaluated and systematically refined through bench top testing and in vivo acute experiments. This project significantly extends the state-of-the-art for microscale neural interfaces by developing a new technology that combines precise, high-resolution neurochemical sensing with high-fidelity neural recording and targeted drug delivery. This technology is likely to provide a solid foundation for developing a new class of implantable device that will enable next-generation, closed-loop neuroprostheses and neuromodulation systems for improved treatments of neurological disease and injury, such as Parkinson's Disease and severe movement disorders.

描述（由申请人提供）：我们的长期研究目标是为中枢神经系统开发长期和高富达微观电气和化学界面的可植入神经技术。 这个为期两年的项目的目的是通过将化学传感器整合在微生物薄膜硅设备上，以开发一种新型的多模式神经探针，该硅设备目前用于神经记录，刺激和显微镜药物注射。 第一个具体目的是开发和表征两种电活性神经化学物质（多巴胺和5-羟色胺）的综合化学感测，以及并发的电生理记录。 主要任务包括开发电极位点材料，将参考电极集成到探针基板上，并表征“电极位点间距，以进行并发神经化学感应和电生理记录。 第二个具体目的是开发干旱的特征，表征两种非电动神经化学物质（乙酰胆碱和谷氨酸）的整合化学感应，以及同时的电生理记录以及药理剂的微流体递送。 该目标的主要任务包括开发聚合物井和导电聚合物涂层，以夹带酶和传感器性能。 在这两个目标中，将通过基准测试和体内急性实验对多模式设备进行定量评估并系统地进行完善。 该项目通过开发一种将精确的高分辨率神经化学传感与高前进性神经记录和靶向药物输送相结合的新技术，可以显着扩展微观神经界面的最新技术。 这项技术可能为开发新的植入式装置提供了坚实的基础，该设备将使下一代，闭环神经预测和神经调节系统改善神经系统疾病和损伤的治疗方法，例如帕金森氏病和严重的运动障碍。