Multi-keV x-ray microscopy facility for bio-imaging

用于生物成像的多 keV X 射线显微镜设备

• 批准号: 7056721
• 负责人: PIERO A PIANETTA
• 金额: $ 79.09万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056721
• 关键词: X ray microscopy; bioimaging /biomedical imaging; biomedical facility; bone imaging /visualization /scanning; contrast media; infection; laboratory mouse; macromolecule; molecular /cellular imaging; scanning electron microscopy; technology /technique development; three dimensional imaging /topography; wound

DESCRIPTION (provided by applicant): We propose to develop a zone plate based, multi-keV x-ray imaging facility at the Stanford Synchrotron Radiation Laboratory (SSRL) that will be capable of imaging thick hydrated biological specimens with 20 nm resolution and provide three dimensional images using tomographic reconstruction. By using x-ray phase contrast imaging as well as contrasting agents and specific labels such as immunogold, quantum dots, or other labeling techniques developed for electron microscopy, the instrument will be a tool for mapping specific molecules in cells or cells in tissues. With the anticipated improvements in zone plate optics, we expect the resolution to ultimately reach or exceed 10 rim. This instrument is meant to be used by the biomedical research community at large, and therefore in its construction we will demonstrate its efficacy on two radically different biological problems: (1) mapping microbes and macromolecules in wound tissue and (2) determining the nanostructure of hydrated bone. This proposal combines the expertise of three collaborating organizations: Prof. P. Pianetta and Dr. K. Luening from SSRL with extensive experience in a wide range of x-ray techniques based on synchrotron radiation sources; Dr. E. Almeida (UCSF, NASA Ames Research Center) and Prof. M. van der Meulen (Cornell University) who are leaders in studies of the nanostructure of bone; and Dr. J. Trent (NASA Ames Research Center), an accomplished microbiologist, who has worked extensively in imaging microbes and macromolecules and currently heads a research team studying the microbial ecology of chronic wounds. The microscope will be purchased from Xradia Inc., a leader in developing nondestructive high-resolution x-ray imaging solutions using multi-keV x-rays produced by a laboratory x-ray source. Dr. Wenbing Yun, founder and president of Xradia, will serve as a consultant on the project to develop the techniques needed for achieving high resolution 2D and 3D images in phase contrast for real biological specimens.

描述（由申请人提供）：我们建议在斯坦福同步子辐射实验室（SSRL）开发基于区域板的多键X射线成像设施，该设施将能够成像具有20 nm分辨率的较厚水合的生物样品，并使用三维图像提供三维图像。通过使用X射线相对比成像以及对比鲜明的剂和特定标签，例如用于电子显微镜开发的免疫金，量子点或其他标记技术，该仪器将是绘制特定分子或组织中细胞中特定分子的工具。随着区域板光学元件的预期改进，我们预计该分辨率最终达到或超过10篮板。该仪器本来要被整个生物医学研究界使用，因此，在其构建中，我们将证明其在两个根本不同的生物学问题上的功效：（1）绘制伤口组织中的微生物和大分子，以及（2）确定水合骨的纳米结构。该提案结合了三个合作组织的专业知识：SSRL的P. Pianetta教授和K. Luening博士以及基于同步辐射来源的各种X射线技术的丰富经验； E. Almeida博士（UCSF，NASA AMES研究中心）和M. van der Meulen教授（康奈尔大学）是骨骼纳米结构研究的领导者；一位成熟的微生物学家J. Trent（NASA AMES研究中心）J. Trent博士（NASA Ames Research Center）在成像微生物和大分子中工作，目前负责研究慢性伤口微生物生态学的研究小组。显微镜将从Xradia Inc.购买，Xradia Inc.是使用实验室X射线源生产的多键X射线开发非破坏性高分辨率X射线成像解决方案的领导者。 Xradia的创始人兼总裁Wenbing Yun博士将担任该项目的顾问，以开发实现实际生物标本相比的高分辨率2D和3D图像所需的技术。