Core-C-chronic hypoxia model

Core-C-慢性缺氧模型

• 批准号: 6910148
• 负责人: George Lister
• 金额: $ 10.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-17 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6910148
• 关键词: biomedical facility; brain; disease /disorder model; experimental designs; heart; hypercapnia; hypoxia; kidney; model design /development; tissue /cell preparation; training

This Hypoxia-Hypercapnia Core will serve the 4 Projects in the Program in various ways. Core C will provide mice (or rats) that have been exposed to hypoxia or hypercapnia (or both) in a standardized and consistent way. Moreover, the Core will promote interactions among the investigators and help generate new avenues of collaborative investigation because of the use of identical models to study mechanisms for response and adaptation to long-term and graded stress. The aims of this Core Facility are as follows: 1. To ensure a consistent experimental model of chronic hypoxia or hypercapnia or both for use in all four projects; 2. To train technicians and postdoctoral and help in tissue dissection and collection for all investigators whether brain, heart or kidney; 3. To continue to characterize the chronic hypoxia-hypercapnia model in order to better understand the implications of novel findings that arise during the course of this program; 4. To develop comparable models of chronic hypoxia or hypercapnia in mice with genetic alterations (e.g. Knock-out such as NHE-/-) to propose specific mechanisms for adaptation or injury; 5. To help investigators in the design of experiments requiring chronic exposure of cells in culture for an extended period of time in hypoxia, hypercapnia or both.

这种缺氧杂质的核心将以各种方式为该计划的4个项目提供服务。核心C将以标准化且一致的方式提供已暴露于缺氧或高脑（或两者）的小鼠（或大鼠）。此外，核心将促进研究人员之间的互动，并帮助产生合作调查的新途径，因为使用相同的模型来研究响应和适应长期和适应的机制 分级应力。 该核心设施的目的如下： 1。确保慢性缺氧或高碳酸盐或两者都使用的一致实验模型 在所有四个项目中； 2。培训技术人员和博士后，并帮助所有人的组织解剖和收集 调查人员是大脑，心脏还是肾脏； 3。继续表征慢性低氧蛋白酶模型，以便更好 了解该计划过程中出现的新发现的含义； 4。开发具有遗传的小鼠的慢性缺氧或高碳酸疾病的可比模型 改变（例如敲除nhe - / - ）提出适应或 受伤; 5。帮助调查人员设计需要长期暴露细胞中的实验的设计 在缺氧，高碳酸盐或两者兼而有之长时间的培养时间。