Mechanisms of hormone-dependent Rho activation

激素依赖性 Rho 激活机制

基本信息

  • 批准号:
    7039095
  • 负责人:
  • 金额:
    $ 24.89万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-07-01 至 2009-04-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Precise temporal and spatial control over cell shape changes and cell rearrangements are essential for the development of all metazoans. Endocrine signaling provides a critical level of temporal control that additionally aids in the coordination of morphogenetic events. The elongation and eversion of Drosophila leg imaginal discs provide an ideal system for defining the molecular mechanisms of hormone-regulated tissue morphogenesis. A pulse of the steroid hormone ecdysone triggers the transformation of an epithelial sac into an immature adult leg through coordinated changes in cell shape. Previous studies indicate that ecdysone-dependent leg morphogenesis requires signaling through the Rho 1 small GTPase. Although the direct effects that Rho 1 signaling has on the actin cytoskeleton are well established, little is known about the mechanisms that control Rho 1 activity in a developmental context. Understanding these control mechanisms for Rho 1 signaling are critical since perturbation of Rho activity during vertebrate embryonic development can lead to incomplete neural tube closure and heart defects. Furthermore, dysregulation of Rho signaling at later stages of development may result in tumor progression and metastasis. The overall goal of the proposed research is to define the morphogenetic pathway of leg development in Drosophila, from an initiating signal that provides temporal specificity, through a Rho 1 intracellular signaling cascade, to the molecules that apply chemomechanical forces to the actin cytoskeleton. This will be accomplished through the development of methods to monitor Rho 1 activation in imaginal discs undergoing morphogenesis. These methods will be used to examine the activation of Rho 1 by ecdysone. In addition, whole Drosophila genome microarray chips will be probed to identify genes induced by ecdysone at the onset of leg morphogenesis, and simultaneous genetic screens will be conducted to identify Rho 1-interacting genes. It is expected that these genetic and molecular screens will converge to identify key genes that regulate Rho 1 signaling and leg morphogenesis, providing a foundation for understanding how Rho 1 is regulated during a developmental program in an intact living organism, as well as providing insights into how Rho signaling can be subverted to a pathological state.
描述(由申请人提供):精确的时间和空间控制细胞形状变化和细胞重排对于所有后生动物的发展至关重要。内分泌信号传导提供了关键的时间控制水平,该水平还有助于形态发生事件的协调。果蝇腿的想象盘的伸长和延伸为定义激素调节的组​​织形态发生的分子机制提供了理想的系统。类固醇激素ecdysone的脉搏通过细胞形状的协调变化触发了上皮囊到不成熟的成年腿的转化。先前的研究表明,依赖ecdysone的腿形态发生需要通过Rho 1小GTPase发出信号。尽管Rho 1信号传导对肌动蛋白细胞骨架的直接影响已经很好地确定,但对在发育环境中控制RHO 1活性的机制知之甚少。了解这些对RHO 1信号传导的控制机制至关重要,因为脊椎动物胚胎发育过程中RHO活性的扰动可能会导致神经管闭合和心脏缺陷。此外,在后期发育阶段,RHO信号传导失调可能导致肿瘤进展和转移。拟议的研究的总体目标是定义果蝇中腿部发育的形态发生途径,从提供时间特异性的启动信号到Rho 1细胞内信号级联级联到分子,这些分子将化学力力量施加到肌动蛋白细胞骨架上。这将通过开发方法来监测发生形态发生的想象盘中Rho 1激活的方法来实现。这些方法将用于检查ecdysone的Rho 1的激活。此外,将探测整个果蝇基因组微阵列芯片,以鉴定腿部形态发生时ecdysone诱导的基因,并将进行同时进行遗传筛选以鉴定RHO 1相互作用基因。预计这些遗传和分子筛选将收敛以识别调节RHO 1信号传导和腿部形态发生的关键基因,从而为理解完整生物的发育程序中如何调节Rho 1的基础,并提供有关如何将Rho信号颠覆到病理状态的洞察力。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ROBERT E WARD其他文献

ROBERT E WARD的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ROBERT E WARD', 18)}}的其他基金

Mechanisms of hormone-dependent Rho activation
激素依赖性 Rho 激活机制
  • 批准号:
    6812969
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of hormone-dependent Rho activation
激素依赖性 Rho 激活机制
  • 批准号:
    7218684
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of hormone-dependent Rho activation
激素依赖性 Rho 激活机制
  • 批准号:
    7423903
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
FUNCTIONS OF RHO: REGULATOR OF DEVELOPMENT AND METASTASIS
RHO 的功能:发育和转移的调节因子
  • 批准号:
    7011665
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of hormone-dependent Rho activation
激素依赖性 Rho 激活机制
  • 批准号:
    6910795
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of Hormone-Induced Morphogenesis
激素诱导的形态发生机制
  • 批准号:
    6405587
  • 财政年份:
    2001
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of Hormone-Induced Morphogenesis
激素诱导的形态发生机制
  • 批准号:
    6526251
  • 财政年份:
    2001
  • 资助金额:
    $ 24.89万
  • 项目类别:

相似海外基金

Regulation of Polyglutamine Aggregation and Toxicity
聚谷氨酰胺聚集和毒性的调节
  • 批准号:
    6980475
  • 财政年份:
    2006
  • 资助金额:
    $ 24.89万
  • 项目类别:
Adhesion dynamics in Drosophila border cell migration
果蝇边缘细胞迁移的粘附动力学
  • 批准号:
    6866989
  • 财政年份:
    2005
  • 资助金额:
    $ 24.89万
  • 项目类别:
Adhesion dynamics in Drosophila border cell migration
果蝇边缘细胞迁移的粘附动力学
  • 批准号:
    7010356
  • 财政年份:
    2005
  • 资助金额:
    $ 24.89万
  • 项目类别:
Adhesion dynamics in Drosophila border cell migration
果蝇边缘细胞迁移的粘附动力学
  • 批准号:
    7289515
  • 财政年份:
    2005
  • 资助金额:
    $ 24.89万
  • 项目类别:
Mechanisms of hormone-dependent Rho activation
激素依赖性 Rho 激活机制
  • 批准号:
    6812969
  • 财政年份:
    2004
  • 资助金额:
    $ 24.89万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了