Mechanisms of Hormone-Induced Morphogenesis

激素诱导的形态发生机制

• 批准号: 6526251
• 负责人: ROBERT E WARD
• 金额: $ 4.65万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6526251
• 关键词: Drosophilidae; biological signal transduction; cell migration; confocal scanning microscopy; developmental genetics; embryo /fetus protein; fluorescent dye /probe; genetic mapping; genetic regulation; genetic transcription; guanine nucleotide binding protein; histogenesis; hormone regulation /control mechanism; imaginal disc; immunofluorescence technique; leg; metamorphosis; molecular cloning; morphology; morphometry; mutant; northern blottings; protein structure function; reporter genes; transcription factor

DESCRIPTION (provided by applicant) Cell shape changes and whole tissue movements are essential for the development of all metazoans. These morphogenetic processes must be precisely coordinated within the organism for development to proceed normally. The proposed research sets out to explore the hormonal regulation of imaginal disc morphogenesis during Drosophila metamorphosis. Drosophila provides an ideal model system for defining the spatial and temporal control of imaginal disc morphogenesis. Since the signal initiating this developmental event is known and there are vast resources available for gene discovery. In the proposed experiments, screens will be performed to identify genes required for imaginal disc morphogenesis. Mutants identified in these screens will be phenotypically characterized and a subset of the corresponding genes will be cloned and further analyzed for specific functions during imaginal disc morphogenesis. The proposed screens will provide a means to uncover the pathway that connects the hormonal signal to the force-generating molecules required for morphogenesis. In addition, the mechanisms that are defined by this research will provide insight into the regulation of morphogenesis in other higher organisms.

描述（由申请人提供） 细胞形状的变化和整个组织的运动对于发育至关重要 所有后生动物中。这些形态发生过程必须精确协调 使发育正常进行。拟议的研究 着手探索成象盘形态发生的激素调节 果蝇变态期间。果蝇提供了一个理想的模型系统 定义成象盘形态发生的空间和时间控制。自从 启动这一发育事件的信号是已知的，并且有大量的信号 可用于基因发现的资源。在提议的实验中，屏幕 将进行以确定成虫盘形态发生所需的基因。 在这些筛选中鉴定出的突变体将进行表型表征并 相应基因的子集将被克隆并进一步分析 成虫盘形态发生过程中的特定功能。提议的屏幕 将提供一种方法来揭示连接荷尔蒙信号的途径 形态发生所需的力产生分子。此外， 本研究定义的机制将提供对 其他高等生物形态发生的调节。