Brain Damage and Recovery of Function in the Adult System

成人系统的脑损伤和功能恢复

• 批准号: 7096064
• 负责人: MEI-FANG CHENG
• 金额: $ 24.63万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-13 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7096064
• 关键词: Aves; appetite regulatory center; auditory stimulus; behavior test; brain injury; bromodeoxyuridine; central neural pathway /tract; experimental brain lesion; functional ability; immunocytochemistry; longitudinal animal study; luteinizing hormone; molecular dynamics; nervous system regeneration; neural information processing; neurogenesis; neuronal transport; neurotoxins; nucleosides; radioimmunoassay; social behavior; stimulus /response; surgery; testosterone

DESCRIPTION (provided by applicant): Our long-term goal is to determine how to help adult victims of brain injury recover the function of brain areas damaged by stroke or other afflictions. To that end, the goal of this study is to determine the mechanisms that underlie the recovery of function after damage to the adult brain. The present proposal builds upon two conceptually complementary observations. We have observed that the recovery of complex courtship behavior (the nest coo) after bilateral hypothalamic lesion in the adult male ring dove is facilitated when a male ring dove is housed with a female. Secondly, after electrolytic lesions of the adult hypothalamus, newborn neurons were detected at the lesion site concurrent with recovery at the lesion site of units exhibiting normal firing responses to female nest coo stimulation and behavioral recovery 2-3 months after lesion. These observations suggest an alternative explanation to the widely accepted principle of recovery of function. Recovery might be mediated not only by existing, undamaged neurons, but also by lesion-induced new neurons recruited into the network of reorganization. We test the hypothesis that lesion-induced new neurons are involved in the recovery of physiological activity and nest coo behavior by blocking neurogenesis and observing the effects on recovery (Aim I) and the determining whether the new neurons are biologically functional, namely, whether they are integrated into the network of nest coo behavior. This will be tested by measuring endocrine output associated with the nest coo behavior, and by determining axonal connections of new neurons using combined immunohistochemistry of tract tracing and neuronal markers (Aim II). In sum, the present proposal seeks to determine the role of lesion-induced neurogenesis in the context of the recovery of function in the mature dove brain.

描述（由申请人提供）：我们的长期目标是确定如何帮助脑损伤的成年受害者恢复受中风或其他痛苦损坏的大脑区域的功能。为此，这项研究的目的是确定损害成人大脑后功能恢复的机制。本提案建立在两个概念上的互补观察基础上。我们已经观察到，当雄性环鸽子与女性饲养时，在成年男性环鸽子双丘脑病变后的复杂求爱行为（巢穴）的恢复。其次，在成年下丘脑的电解病变后，在病变部位发现了新生神经元，并在病变的病变部位恢复，在病变后2-3个月恢复了对女性巢穴刺激和行为恢复正常的反应。这些观察结果表明了对功能恢复的广泛接受原则的另一种解释。恢复不仅可能是由现有未损坏的神经元介导的，而且还可以由病变引起的新神经元招募到重组网络中。我们通过阻止神经发生并观察对恢复的影响（AIM I）以及确定新神经元是否在生物学上功能性（不可能）整合到Nest COO行为的网络中，从而检验了病变诱导的新神经元与生理活性和巢COO行为的恢复有关的假设。这将通过测量与Nest COO行为相关的内分泌输出，以及使用道跟踪和神经元标记的联合免疫组织化学确定新神经元的轴突连接（AIM II）来测试这一点。总而言之，本提案旨在确定病变诱导的神经发生在成熟鸽子大脑中功能的恢复背景下的作用。