Regulation of Angiopoietin mRNA Stability

血管生成素 mRNA 稳定性的调节

• 批准号: 7095875
• 负责人: JOHN VOGEL
• 金额: $ 12.24万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7095875
• 关键词: RNA binding protein; RNA biosynthesis; affinity chromatography; angiogenesis; angiopoietins; animal genetic material tag; biological signal transduction; cell growth regulation; complementary DNA; enzyme activity; gel mobility shift assay; genetic regulatory element; human genetic material tag; messenger RNA; phosphatidylinositol 3 kinase; polymerase chain reaction; protein binding; protein purification; protein sequence; transcription factor; vascular endothelium; vascular smooth muscle; western blottings

DESCRIPTION (provided by applicant): A five year training program of integrated didactic and laboratory experiences to facilitate the development of an academic career involving molecular biology based research. The principle investigator has successfully completed a residency in Anatomic and Clinical Pathology and is currently in a two-year Molecular Genetic Pathology Fellowship. To build toward the goal of obtaining a tenure track position in an academic setting, and to combine a career in both basic research and academic medicine, the choice was made to obtain education and experience in molecular biological research involving angiogenesis and its role in diseases of the eye. This goal will be accomplished through a challenging research proposal focused on signal transduction pathways associated with Angiopoietin-2 (Ang2) mRNA stability. Ang2 is critically involved in blood vessel stability, and is involved in ocular neovascularization. Preliminary data indicate that Ang2 is significantly regulated post-transcriptionally, and that phosphoinositide-3-kinase (PI3K) activity plays a critical role in Ang2 mRNA stability. We will identify cis-acting elements in Ang2 mRNA and the associated trans-acting factors involved in PI3K-mediated Ang2 mRNA stability. Drs. Anderson (Mentor - Chair of the Department of Cell Biology) and Howard (Co-Mentor - Associate Professor of Cell Biology) have extensive research experience in the fields of eye disease (including the role of PI3K in ocular disease) and angiogenesis (including the regulation angiopoietins), respectively. The University of Oklahoma Health Sciences Center is strongly committed to ocular research through the Dean McGee Eye Institute. The facilities available to the principal investigator provide the modem equipment necessary for this level of molecular-based research.

描述（由申请人提供）：一项为期五年的综合教学和实验室经验培训计划，以促进涉及基于分子生物学研究的学术职业的发展。原则研究者已成功完成了解剖学和临床病理学的居留权，目前处于两年的分子遗传病理研究金。为了建立在学术环境中获得终身轨道位置，并结合基础研究和学术医学的职业的目标，选择了涉及血管生成及其在眼睛疾病中的作用的分子生物学研究的教育和经验。该目标将通过一项具有挑战性的研究建议来实现，该研究的重点是与血管生成素-2（ANG2）mRNA稳定性相关的信号转导途径。 Ang2与血管稳定性有关，并参与眼部新血管形成。初步数据表明，ANG2在转录后受到显着调节，并且磷酸肌醇3-激酶（PI3K）活性在ANG2 mRNA稳定性中起关键作用。我们将确定ANG2 mRNA中的顺式作用元件以及PI3K介导的ANG2 mRNA稳定性所涉及的相关的跨作用因子。博士。安德森（导师 - 细胞生物学系主任）和霍华德（Co -Intor-细胞生物学副教授）在眼科疾病领域（包括PI3K在眼部疾病中的作用）和血管生成（包括调节血管生成素）具有丰富的研究经验。俄克拉荷马大学健康科学中心通过Dean McGee眼科研究所强烈致力于眼部研究。主要研究人员可用的设施提供了这一基于分子的研究所需的调制解调器设备。