Detection of Bioterrorism Agents with PM Technology

利用 PM 技术检测生物恐怖主义制剂

• 批准号: 7013145
• 负责人: BARRY RONALD BOCHNER
• 金额: $ 44.29万
• 依托单位: BIOLOG, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7013145
• 关键词: Detection; Bioterrorism; Agents; PM; Technology

DESCRIPTION (provided by applicant): Laboratories doing routine microbiology now must have tools that allow them to recognize and identify dangerous pathogens that could appear as part of their normal workflow. They need this capability to identify incipient bioterrorism attacks, to save lives of stricken people by rapid diagnosis, and to prevent the laboratory workers themselves from dangerous and uncontrolled exposures. Our proposal provides novel yet practical solutions to the real problems faced by microbiologists right now. Biolog will develop a highly advanced, bacterial identification system that can be used by any clinical microbiology laboratory to identify over 800 different bacterial species including bioterrorism microorganisms that could be used in an attack against humans or U.S. agriculture. This development is enabled by Biolog's newly developed, proprietary platform technology, Phenotype MicroArrays (PMs). PMs constitute a new genomic scale technology that gives unprecedented detailed information about the properties of live cells allowing scientists to test 2000 properties of a microbial cell simultaneously in a proprietary recording instrument called the OmniLog. The core technology is an image analysis system that detects changes in respiration of cells grown under different conditions. Because a large number of parameters are measured, an information-rich colorimetric pattern is generated for each bacterium, constituting a fingerprint of that bacterium. By testing a broad and representative collection of bacterial species in an automated high-throughput program, we will rapidly build a detailed database on phenotypic properties common to each bacterial species as well as properties that distinguish each species from its closest relatives. The database will be used to generate test panels capable of identifying over 800 species of both gram-negative and gram-positive bacteria in a single test. This will improve the simplicity, speed, and accuracy of bacterial identification far beyond the technology available to medical labs at this time. We will also develop new, highly selective and specific enrichment and culture media that will allow investigators to monitor for bioterrorism microorganisms in the environment using standard, inexpensive, low tech agar plates.

描述（由申请人提供）： 现在，从事常规微生物学的实验室必须具有使他们识别并识别可能是正常工作流程的一部分的危险病原体的工具。他们需要这种能力来识别初期的生物恐怖袭击，通过快速诊断来挽救受震惊的人的生命，并防止实验室工人自己免受危险和不受控制的暴露。我们的建议为现在微生物学家面临的真正问题提供了新颖而实用的解决方案。生物学将开发出高度先进的细菌鉴定系统，任何临床微生物学实验室都可以使用，以鉴定800多种不同的细菌物种，包括生物恐怖的微生物，可用于攻击人类或美国农业。生物学新开发的专有平台技术，表型微阵列（PMS）实现了这一开发。 PMS构成了一种新的基因组量表技术，该技术提供了有关活细胞特性的前所未有的详细信息，使科学家可以在称为Omnilog的专有记录仪器中同时测试微生物细胞的2000性能。核心技术是一个图像分析系统，可检测在不同条件下生长的细胞的呼吸变化。由于测量了大量参数，因此为每个细菌生成了富含信息的比色性图案，构成了该细菌的指纹。通过在一个自动高通量程序中测试细菌物种的广泛而代表性的收集，我们将迅速构建有关每个细菌物种常见的表型特性的详细数据库，以及将每个物种与最接近的亲属区分开的特性。该数据库将用于生成能够在单个测试中识别800种革兰氏阴性和革兰氏阳性细菌的测试面板。这将提高细菌识别的简单性，速度和准确性，远远超出了此时医疗实验室的技术。我们还将开发新的，高度选择性和特定的丰富和培养基，使研究人员使用标准，廉价，低技术琼脂板在环境中监测生物恐怖主义微生物。