CORE--ANIMAL CORE

核心--动物核心

• 批准号: 6951264
• 负责人: David Alan Kass
• 金额: $ 20.31万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951264
• 关键词: animal care; animal colony; bioenergetics; biomechanics; biomedical facility; blood pressure; disease /disorder model; dogs; echocardiography; electronic pacemaker; electrophysiology; heart conduction system; heart contraction; heart disorder; heart electrical activity; heart failure; heart surgery; hypertrophic myocardiopathy; tissue /cell preparation; veterinary medicine

The animal and tissue procurement core is responsible for generating the canine model of cardiac dyssynchrony and/or tachypacing-induced dilated cardiomyopathy. Adult mongrel dogs weighing approximately 25 kg are employed in this study. Dyssynchrony is generated by radiofrequency ablation of the left bundle (or right bundle in a sub-set of studies). In addition, dyssynchrony can be induced by single-point pacing of the RV or LV free-wall - and this variant can also be utilized for some of the molecular/cellular analyses. Cardiac failure is induced by tachycardia pacing over a period of 3-6 weeks depending on the protocol. Cardiac resynchronization is achieved by converting 3-weeks of rapid atrial pacing in the setting of an established conduction delay - with 3-4 weeks of bi-ventricular pacing of left ventricular free wall and right ventricles. The primary aims of this Core are to 1) Perform the surgical and electrophysiologic procedures required to induce dyssynchrony with and without cardiac failure. Provide chronic instrumentation for mechanical and energetic analyses as required by the individual projects. 2) Maintain all chronic care of the animal, providing serial physiologic assessment as required, including coordinating echocardiographic analysis, intermittent pressure measurements, etc.. 3) Coordinate veterinary support on a per-need basis to assure optimal care and maintenance of the animals, catheters, repairing fractured or damaged pacing leads, etc.. 4) Perform terminal study - including rapid procurement of myocardial tissues used to wedge and cellular preparations, and rapid processing of frozen materials required for molecular and histochemistry analyses.

动物和组织采购核心负责生成犬类模型 心脏不同步和/或心动过速引起的扩张型心肌病。成年杂种狗 本研究中使用的重量约为 25 公斤。不同步是由以下原因产生的 左束射频消融（或研究子集中的右束）。此外， 右心室或左心室游离壁的单点起搏可以引起不同步，并且这种变体也可以用于一些分子/细胞分析。心力衰竭是由 根据方案，心动过速起搏持续 3-6 周。心脏 再同步是通过将 3 周的快速心房起搏转换为 确定传导延迟 - 对左心室游离壁和右心室进行 3-4 周的双心室起搏。 该核心的主要目标是 1) 执行诱导不同步（有或没有心力衰竭）所需的外科和电生理程序。根据各个项目的要求，提供用于机械和能量分析的长期仪器。 2) 维持对动物的所有长期护理，根据需要提供系列生理评估， 包括协调超声心动图分析、间歇压力测量等。 3) 根据需要协调兽医支持，以确保最佳的护理和维护 动物、导管、修复断裂或损坏的起搏导线等。 4) 进行终端研究——包括快速获取用于楔入的心肌组织 和细胞制剂，以及分子和细胞所需的冷冻材料的快速加工 组织化学分析。