Stress and Body Weight Regulation

压力和体重调节

• 批准号: 7008191
• 负责人: Randall R. Sakai
• 金额: $ 32.98万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7008191
• 关键词: animal colony; behavioral /social science research tag; bioenergetics; blood chemistry; body composition; body weight; calorimetry; ethology; homeostasis; in situ hybridization; laboratory rat; neuroendocrine system; neuropeptides; nutrient intake activity; social dominance; stress; weight control; weight loss

DESCRIPTION (provided by applicant): The regulation of food intake and body weight is a complex interaction of multiple factors. Among these factors is the social milieu in which the animal lives and in particular the social stress that accompanies dominance hierarchies. We utilize a unique animal model of social stress to study how such social hierarchies influence the regulation of food intake and body weight and additionally to uncover the neuroendocrine mechanisms that underlie social influences on body weight. In particular, we found that when animals are placed into a visible burrow system (VBS), we can identify the subordinate (SUB) and dominant (DOM) animals and that SUB animals have reduced body weight when they are in the burrow. The focus of this proposal is to test several hypotheses concerning the mechanism by which social stress in subordinate animals alters the fundamental neuroendocrine circuits involved in the regulation of food intake and body weight. The proposed experiments are critical to a complete understanding of how food intake and body weight are regulated. Importantly, the proposed model offers a novel approach for exploring the complicated but vital relationship between the stress/HPA system and body weight regulation. Specific Aims are: 1) To determine whether the weight loss that is observed in SUB is a result of reduced energy intake and/or increased energy expenditure. In addition, we will determine several endocrine and neurochemical endpoints that are altered by negative energy balance. With several novel control groups for comparison (dominant rats, ad lib fed, pair-fed and body weight-matched), we will be able to determine which major systems interact to produce the observed body weight changes. 2) To determine whether the reduced food intake and/or increased energy expenditure of SUB is secondary to a homeostatic down regulation of the defended amount of body fat, or to a direct catabolic action of stress hormones and neurotransmitters. 3) To test the hypothesis that multiple cycles of chronic stress in the VBS and recovery will produce more severe and enduring changes in body weight regulatory systems than exposure to a single episode. The health significance of this research is clear. Determining how CNS mechanisms involved in the regulation of energy balance are altered by stress is critical to a complete understanding of the co-morbidity of stress related disease states and obesity. Interestingly both stress and food intake regulation share common neurochemical systems and this proposal attempts to define both the interrelatedness as well as the independence of the mechanisms that govern each system. The execution of this proposal will provide insight into the connections between different levels of this regulatory system and thereby give direction to how multiple interventions might best be used to prevent or treat obesity and associated conditions resulting from stress.

描述（由申请人提供）：食物摄入和体重的调节是多种因素的复杂相互作用。这些因素包括动物生活的社会环境，尤其是伴随主导地位的社会压力。我们利用一种独特的社会压力动物模型来研究这种社会等级如何影响食物摄入和体重的调节，除了发现社会对体重影响的神经内分泌机制外。特别是，我们发现，当动物被放入可见的洞穴系统（VBS）中时，我们可以识别下属（sub）和显性（DOM）动物，而亚动物在洞穴中的体重降低。该提案的重点是检验几种关于下属动物社会压力的机制的假设，可以改变与食物摄入和体重调节有关的基本神经内分泌回路。提出的实验对于完全了解食物摄入量和体重的调节至关重要。重要的是，提出的模型提供了一种新颖的方法，用于探索压力/HPA系统与体重调节之间的复杂但至关重要的关系。具体目的是：1）确定SUB中观察到的体重减轻是否是能量摄入减少和/或能量消耗增加的结果。此外，我们将确定几种因负能量平衡而改变的内分泌和神经化学终点。有了几个新颖的对照组进行比较（主要的大鼠，AD LIB喂养，配对和体重匹配），我们将能够确定哪些主要系统相互作用以产生观察到的体重变化。 2）确定子的食物摄入量减少和/或sub的能量消耗是继发性体内脂肪量的减少调节，还是胁迫激素和神经递质的直接分解代谢作用。 3）检验以下假设：VBS和恢复中多个慢性应激周期将产生体重调节系统的更严重和持久的变化，而不是暴露于单个发作。这项研究的健康意义是显而易见的。确定如何通过压力来改变参与能量平衡调节的中枢神经系统机制对于完全理解应激相关疾病状态和肥胖的合并症至关重要。有趣的是，压力和食物摄入调节都具有共同的神经化学系统，该提议试图定义控制每个系统的机制的相互关联性和独立性。该提案的执行将提供有关该监管系统不同级别之间的联系的洞察力，从而为如何最好地使用多种干预措施来预防或治疗压力导致的肥胖和相关状况。