Laboratory Rat Core

实验室大鼠核心

• 批准号: 6850622
• 负责人: PAUL M PLOTSKY
• 金额: $ 15.3万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6850622
• 关键词: animal care; behavioral /social science research tag; biomedical facility; clinical depression; disease /disorder model; early experience; gender difference; hypothalamic pituitary adrenal axis; laboratory rat; mother deprivation

The Rat Model Core (Core 9001) of the Emory University Silvio O. Conte Center for the Neuroscience of Mental Disorders (CCNMD) represents an integral component of the overall Center. This Core will serve as the source of all rodents used by individual preclinical Research Projects 1-4. This Core will produce and characterize variants of a rat early life stress (ELS) model associated with vulnerability to the development of an anxiety-and depression-like syndrome. Personnel will purchase or breed rodents, provide high quality animal care and uniformly applied neonatal manipulation protocols necessary to generate the ELS model, characterize the phenotype of all animals, ascertain the stage of the estrus cycle in adult female animals, and provide experimental manipulations as required by individual preclinical Research Projects within the Center. This ELS model consists of Long Evans rats which are exposed to different rearing conditions from postnatal days (PND) 2-12, including: (1) animal facility reared (AFR) colony controls, (2) brief handling plus 15 min maternal separation (HMS15) handling controls, (3) brief handling plus variable maternal separation (HMS180+). Comparison of data obtained from these standardized rearing conditions among the various basic Research projects of this CCNMD will permit identification of central neurocircuits and intracellular mechanisms which are impacted by early life stress and which give rise to this anxiety- and depression-like syndrome; furthermore, because the animal models will be standardized, regression modeling can be used to elucidate interactions among these CNS systems in the genesis of this condition. In addition, the efficacy of various manipulations or treatments on aspects of the anxiety- and depression-like syndrome will be tested in these models. An important aspect of these animals is comparison with the phenotype and other measures in the nonhuman primate model as well as in the human studies. To this end, Core 9001 will characterize the behavioral and HPA axis stress responsiveness of animals, provide phenotypic screening for anhedonia, anxiety-/fear-like behavior, as well as providing surgical services, resident intruder social defeat exposure, and other protocols as required by the Research Projects. This organization has the advantage of maintaining consistency in animal handling and implementation of all protocols so that each preclinical Research Project receives identically characterized and treated animals for study.

Emory大学Silvio O. Conte神经科学中心（CCNMD）的大鼠模型核心（Core 9001）代表了整个中心的组成部分。该核心将作为单个临床前研究项目1-4使用的所有啮齿动物的来源。该核心将产生并表征与焦虑和抑郁症综合征发展的脆弱性相关的大鼠早期生命应力（ELS）模型的变体。人员将购买或繁殖啮齿动物，提供高质量的动物护理以及统一应用的新生儿操纵方案，以生成ELS模型， 表征所有动物的表型，确定成年雌性动物的发情周期的阶段，并根据中心内的单个临床前研究项目的要求提供实验操作。该ELS模型由长埃文斯大鼠组成，该大鼠暴露于产后天（PND）2-12的不同饲养条件，包括：（1）动物设施饲养（AFR）殖民地控制，（2）简短处理加15分钟的母体分离（HMS15）处理控制，（3）简短处理加上可变的母亲分离（HMS180+）。从这些标准化的饲养条件中获得的数据比较该CCNMD的各种基础研究项目将允许识别受到早期生命压力影响的中央神经环节和细胞内机制，并引起这种焦虑和抑郁症状 综合征；此外，由于动物模型将是标准化的，因此可以使用回归模型来阐明这些条件起源中这些CNS系统之间的相互作用。此外，在这些模型中将测试各种操纵或治疗对焦虑和抑郁症综合征方面的功效。这些动物的一个重要方面是与非人类灵长类动物模型以及人类研究中的表型和其他措施进行比较。为此，Core 9001将表征动物的行为和HPA轴应力反应性，为Anhedonia，焦虑/类似恐惧的行为以及提供手术服务提供表型筛查，居民社交 击败研究项目要求的曝光和其他协议。该组织具有保持动物处理和实施所有方案的一致性的优势，因此每个临床前研究项目都具有相同的表征和治疗的动物进行研究。