CORE--Quantitative Morphology

核心--定量形态学

• 批准号: 6850600
• 负责人: PATRICK R HOF
• 金额: $ 15.93万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6850600
• 关键词: amygdala; animal tissue; bioimaging /biomedical imaging; biomedical facility; biomedical registry /referral center; brain imaging /visualization /scanning; brain mapping; computer simulation; digital imaging; health science research support; human tissue; image processing; magnetic resonance imaging; mathematical model; method development; neural plasticity; postmortem; three dimensional imaging /topography

The Quantitative Morphology Core (QMC) provides technology supports to the members of the Conte Center. The QMC has developed tools to quantify morphologic changes in spines and synapses at the confocal , multiphoton, and electron microscopy levels. These methods are developed to provide to provide the necessary degree of resolution to quantify plasticity-related changes in molecular constituents of synapses, a critical step to link such modifications to information processing. The QMC has developed applications designed to increase the throughput and accuracy, and reduce bias of quantitative analysis of immunogold electron microscopy of synapses as well as new tools to image Lucifer Yellow-injected neurons at high resolution from automatized acquisition of entire cells using confocal and multiphoton data.. These tools involve deconvolution, smoothing, automatic tiling, and 3-dimensional rendition with high level of detail and capacity for morphometric analysis of dendrites at the spine level of resolution. We are also performing mathematical modeling of neuronal complexity and biophysical simulation of the properties of the reconstructed neurons. The QMC was also involved in the development of a new stereologic probe to assess the 3-dimensional spatial distribution of particles in a volume of interest. Recently, the QMC has begun producing high resolution magnetic resonance microscopy (MRM; 9.4 T) datasets of the human amygdala to provide users with a high level of detail digital atlas of the human amygdala to be used as a reference guide for fMRI and PET analyses of live subjects as well as anatomic reference for amygdala nuclear parcellation. These MRM data will also inform the users on the degree of anatomic variation that occurs in the human amygdala.

定量形态学核心（QMC）为Conte中心成员提供技术支持。 QMC 开发了在共焦、多光子和电子显微镜水平上量化棘和突触形态变化的工具。开发这些方法是为了提供必要的分辨率来量化突触分子成分中与可塑性相关的变化，这是将此类修改与信息处理联系起来的关键步骤。 QMC 开发了旨在提高通量和准确性并减少突触免疫金电子显微镜定量分析偏差的应用程序，以及使用共聚焦和多光子自动采集整个细胞来以高分辨率对注射荧光黄的神经元进行成像的新工具这些工具涉及反卷积、平滑、自动平铺和 3 维再现，具有高水平的细节和在脊柱分辨率水平上对树突进行形态分析的能力。我们还对神经元复杂性进行数学建模，并对重建神经元的特性进行生物物理模拟。 QMC 还参与了新型立体探针的开发，以评估感兴趣体积中粒子的 3 维空间分布。最近，QMC 已开始生成人类杏仁核的高分辨率磁共振显微镜（MRM；9.4 T）数据集，为用户提供高水平细节的人类杏仁核数字图谱，用作 fMRI 和 PET 分析的参考指南活体受试者以及杏仁核核分割的解剖参考。这些 MRM 数据还将告知用户人类杏仁核中发生的解剖变异程度。