CORE--Quantitative Morphology

核心--定量形态学

• 批准号: 6850600
• 负责人: PATRICK R HOF
• 金额: $ 15.93万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6850600
• 关键词: amygdala; animal tissue; bioimaging /biomedical imaging; biomedical facility; biomedical registry /referral center; brain imaging /visualization /scanning; brain mapping; computer simulation; digital imaging; health science research support; human tissue; image processing; magnetic resonance imaging; mathematical model; method development; neural plasticity; postmortem; three dimensional imaging /topography

The Quantitative Morphology Core (QMC) provides technology supports to the members of the Conte Center. The QMC has developed tools to quantify morphologic changes in spines and synapses at the confocal , multiphoton, and electron microscopy levels. These methods are developed to provide to provide the necessary degree of resolution to quantify plasticity-related changes in molecular constituents of synapses, a critical step to link such modifications to information processing. The QMC has developed applications designed to increase the throughput and accuracy, and reduce bias of quantitative analysis of immunogold electron microscopy of synapses as well as new tools to image Lucifer Yellow-injected neurons at high resolution from automatized acquisition of entire cells using confocal and multiphoton data.. These tools involve deconvolution, smoothing, automatic tiling, and 3-dimensional rendition with high level of detail and capacity for morphometric analysis of dendrites at the spine level of resolution. We are also performing mathematical modeling of neuronal complexity and biophysical simulation of the properties of the reconstructed neurons. The QMC was also involved in the development of a new stereologic probe to assess the 3-dimensional spatial distribution of particles in a volume of interest. Recently, the QMC has begun producing high resolution magnetic resonance microscopy (MRM; 9.4 T) datasets of the human amygdala to provide users with a high level of detail digital atlas of the human amygdala to be used as a reference guide for fMRI and PET analyses of live subjects as well as anatomic reference for amygdala nuclear parcellation. These MRM data will also inform the users on the degree of anatomic variation that occurs in the human amygdala.

定量形态核心（QMC）为孔戴中心成员提供了技术支持。 QMC开发了量化共聚焦，多光子和电子显微镜水平上刺和突触的形态变化的工具。开发这些方法是为了提供必要的分辨率，以量化突触的分子成分的可塑性相关变化，这是将这种修改与信息处理联系起来的关键步骤。 QMC已开发了旨在提高吞吐量和准确性的应用，并减少了对突触的免疫元电子显微镜的定量分析的偏差，以及新的工具，以自动化整个细胞的高分化，以高分辨率的高分辨率进行整个细胞的高分辨率，这些工具涉及这些工具，这些工具涉及整个细胞。这些工具涉及这些工具。在分辨率的脊柱水平上对树突的形态分析。我们还对重建神经元的性质进行了神经元复杂性和生物物理模拟的数学模型。 QMC还参与了一种新的立体探针的发展，以评估颗粒的3维空间分布。最近，QMC已开始产生高分辨率的磁共振显微镜（MRM; 9.4 t）人类杏仁核的数据集，以为用户提供高度详细的人类杏仁核的数字地图集，以作为FMRI和pet tosements and Anatomic Cression的参考指南和宠物分析。这些MRM数据还将告知用户人杏仁核发生的解剖变异程度。