Egr genes in sympathetic nervous system development

Egr基因在交感神经系统发育中的作用

• 批准号: 6886368
• 负责人: LAURIE CHRISTINE ELDREDGE
• 金额: $ 2.89万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-21 至 2007-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6886368
• 关键词: biological signal transduction; cAMP response element binding protein; developmental genetics; developmental neurobiology; genetic transcription; growth factor; laboratory mouse; neurotrophic factors; predoctoral investigator; regulatory gene; sympathetic nervous system

DESCRIPTION (provided by applicant): Early Growth Response (Egr) genes may have an important role in mammalian sympathetic nervous system development. Egr mediated gene transcription is essential for differentiation of sympathetic nervous system derived PC12 and neuroblastoma cell lines. However, their role in sympathetic neuron development has never been addressed in vivo. We have outlined a training and research program that will: 1) Characterize the sympathetic nervous system defects in Egr3-deficient mice; 2) determine which Egr gene family members are expressed in sympathetic neurons in vivo; and 3) explore the role of signal transduction pathways involving neurotrophin-3 (NT-3) and upstream transcriptional regulators such as CREB in regulating Egr gene expression during sympathetic neuron development. A greater understanding of the signal transduction mechanisms related to growth factor mediated differentiation and survival may provide important insights into the pathogenesis of tumors and neurodegenerative diseases involving sympathetic neurons.

描述（由申请人提供）：早期生长反应（Egr）基因可能在哺乳动物交感神经系统发育中发挥重要作用。 Egr 介导的基因转录对于交感神经系统衍生的 PC12 和神经母细胞瘤细胞系的分化至关重要。然而，它们在交感神经元发育中的作用从未在体内得到解决。我们概述了一项培训和研究计划，该计划将： 1) 表征 Egr3 缺陷小鼠的交感神经系统缺陷； 2)确定哪些Egr基因家族成员在体内交感神经元中表达； 3）探索涉及神经营养蛋白-3（NT-3）和上游转录调节因子（如CREB）的信号转导通路在交感神经元发育过程中调节Egr基因表达的作用。更好地了解与生长因子介导的分化和存活相关的信号转导机制可能会为涉及交感神经元的肿瘤和神经退行性疾病的发病机制提供重要的见解。