MInnesota Human MAPC Training Grant

明尼苏达州人类 MAPC 培训补助金

• 批准号: 6770707
• 负责人: CATHERINE M VERFAILLIE
• 金额: $ 12万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-07 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6770707
• 关键词: MInnesota; Human; MAPC; Training; Grant

DESCRIPTION (provided by applicant): We identified a rare progenitor cell in BM of humans, mice and rats, which we have termed Multipotent Adult Progenitor Cell or MAPC. MAPC proliferate without senescence and single MAPC differentiate into most mesodermal cell types, neuroectoderm-like and hepatocyte-like cells. MAPC do not form tumors when infused in immunodeficient mice, but differentiate in response to local cues into hematopoietic cells and epithelium of lung, intestine and liver. When injected in the blastocyst, single MAPC contribute to all tissues of the chimeric animal. MAPC may also be capable of differentiation to pancreatic endocrine cells. We can routinely isolate MAPC from >60% of normal human BMs, as well from BMs from C57B16 and 129 mice, maintaining MAPC pluripotent requires that cells are maintained at tightly controlled densities. It is our experience that new investigators learning MAPC culture techniques commonly require several weeks of hands-on experience to gauge the density at which cells should be maintained and the time of cell passaging. As the development of MAPC-based therapies, such as derivation of beta cells, will require that a large number of investigators have access to MAPC and gain knowledge of MAPC culture techniques, we propose to establish a training program for NIH-funded investigators with as goal to disseminate human as well as rodent MAPC, and human and rodent MAPC culture technology. To achieve this goal, we propose to accommodate training of investigators in MAPC culture techniques for a 4-6 week period at the University of Minnesota. Investigators will be trained in the selection of MAPC from fresh human, mouse and / or rat bone marrow, maintenance of established MAPC cell lines, and differentiation of MAPC towards mesodermal, endodermal and neuroectodermal cell types. Funding is requested for support of a technician as well as supplies to establish, maintain and differentiate MAPC, to train 2 investigators for 4-6 weeks at a time.

描述（由申请人提供）： 我们在人类、小鼠和大鼠的骨髓中发现了一种罕见的祖细胞，我们将其称为多能成体祖细胞或 MAPC。 MAPC 增殖而不衰老，并且单个 MAPC 分化为大多数中胚层细胞类型、神经外胚层样细胞和肝细胞样细胞。 当注入免疫缺陷小鼠体内时，MAPC 不会形成肿瘤，但会根据局部信号分化为造血细胞以及肺、肠和肝的上皮细胞。当注射到囊胚中时，单个 MAPC 对嵌合动物的所有组织都有贡献。 MAPC 还可能能够分化为胰腺内分泌细胞。 我们可以常规地从 > 60% 的正常人 BM 以及 C57B16 和 129 小鼠的 BM 中分离出 MAPC，维持 MAPC 多能性需要将细胞维持在严格控制的密度下。根据我们的经验，学习 MAPC 培养技术的新研究者通常需要几周的实践经验来衡量细胞应维持的密度和细胞传代的时间。由于基于 MAPC 的疗法（例如 β 细胞的衍生）的开发将需要大量研究人员能够使用 MAPC 并获得 MAPC 培养技术的知识，因此我们建议为 NIH 资助的研究人员建立一个培训计划，其中包括：目标是传播人类和啮齿动物 MAPC，以及人类和啮齿动物 MAPC 培养技术。为了实现这一目标，我们建议在明尼苏达大学对研究人员进行为期 4-6 周的 MAPC 培养技术培训。研究人员将接受从新鲜人、小鼠和/或大鼠骨髓中选择 MAPC、维持已建立的 MAPC 细胞系以及将 MAPC 分化为中胚层、内胚层和神经外胚层细胞类型的培训。需要资金支持一名技术人员以及物资，以建立、维护和区分 MAPC，一次培训 2 名调查员 4-6 周。