Fourier Transform Mass Spec Proteomics and Genomics

傅里叶变换质谱蛋白质组学和基因组学

• 批准号: 6804239
• 负责人: Ian Alexander Blair
• 金额: $ 77.16万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6804239
• 关键词: biomedical equipment purchase; functional /structural genomics; ion cyclotron resonance spectrometry; mass spectrometry; proteomics; structural biology

DESCRIPTION (provided by applicant): Fourier Transform Mass Spectrometry for Proteomics and Genomics: Fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS) instruments are relatively new to proteomics and genomics research. FTICR/MS rapidly provides highly accurate mass determinations of peptides derived from protease digests of intact proteins. Thus, FTICR-MS systems provide unparalleled capabilities for rapid fingerprinting of known proteins as well as the characterization of new proteins. They can also be used for the unequivocal characterization of covalently modified DNA. Thus, such instruments are essential for state-of-the-art of research in the area of proteomics research when only trace amounts of material are available. The Thermo Finnigan system (LTQ FT) can utilize a variety of ionization techniques: ESI, APCI, APPI or MALDI. Maintenance of the API source, as well as switching between AP ionization methods, is vent-free. Ions are transferred by octapole and "square" quadrupole lenses into a novel ion trap. The linear ion trap is a fully operational mass spectrometer, which can store, isolate, and fragment ions and then send them either to the ICR cell via axial ion ejection for further analysis or to an off-axis SEM detector. The linear ion trap is a unique ion preparation and injection system for FTICR-MS because it has much greater ion storage capacity than conventional 3D ion trap devices. Ions from the ion trap are transferred as discrete bundles into the ICR cell, via octapole ion guides. These octapole ion guides are designed to enable very efficient differential pumping between the ion trap and the ICR cell. The FTICR analyzer is built around an actively shielded superconducting magnet with a field strength of 7 Tesla. The investigators involved in this proposal have research programs that represent four major research areas within the University of Pennsylvania. These programs are highly interactive. Dr. Blair as Director of the Center for Cancer Pharmacology and Scientific Director of the Genomics Institute Proteomics Resource is a member the Cancer Center, the Center for Experimental Therapeutics, and the Institute for Medicine and Engineering. Dr. FitzGerald as Director of the Center for Experimental Therapeutics and a member of the Institute for Medicine and Engineering has extensive interactions with Dr. Davies, Director of the Institute for Medicine and Engineering and Dr. Rader, Director, Lipid Clinics; Assistant Director, General Clinical Research Center through an existing program project grant and a SCOR grant. Three additional investigators from these research programs (Drs. Eberwine, Ischiropoulos, and Roos) will require access to the instrument on a more limited basis. The proposed research on the two major degenerative disease of aging, cancer and cardiovascular disease, will involve a highly interactive approach. This will permit advances made by one investigator to be readily incorporated into research of the other investigators. Thus, while each investigator's research proposal stands alone in scientific value and integrity, the added value of this combined proposal is substantial.

描述（由申请人提供）： 用于蛋白质组学和基因组学的傅里叶变换质谱分析：傅里叶变换离子回旋共振质谱 (FTICR-MS) 仪器对于蛋白质组学和基因组学研究来说相对较新。 FTICR/MS 可快速对源自完整蛋白质的蛋白酶消化物的肽进行高精度质量测定。因此，FTICR-MS 系统为已知蛋白质的快速指纹识别以及新蛋白质的表征提供了无与伦比的功能。它们还可用于明确表征共价修饰的 DNA。因此，当只有微量材料可用时，此类仪器对于蛋白质组学研究领域的最先进研究至关重要。 Thermo Finnigan 系统 (LTQ FT) 可利用多种电离技术：ESI、APCI、APPI 或 MALDI。 API 源的维护以及 AP 电离方法之间的切换都是无排气的。离子通过八极和“方形”四极透镜转移到新型离子阱中。线性离子阱是一款功能齐全的质谱仪，它可以存储、分离和破碎离子，然后通过轴向离子喷射将它们发送到 ICR 单元以进行进一步分析，或者发送到离轴 SEM 检测器。线性离子阱是 FTICR-MS 的独特离子制备和注入系统，因为它比传统 3D 离子阱设备具有更大的离子存储容量。来自离子阱的离子通过八极离子导向器以离散束的形式转移到 ICR 单元中。这些八极离子导向器旨在实现离子阱和 ICR 单元之间非常高效的差动泵送。 FTICR 分析仪围绕磁场强度为 7 特斯拉的主动屏蔽超导磁体构建。参与该提案的研究人员的研究项目代表宾夕法尼亚大学的四个主要研究领域。这些程序具有很强的互动性。 Blair 博士作为癌症药理学中心主任和基因组学研究所蛋白质组学资源科学主任，是癌症中心、实验治疗中心以及医学和工程研究所的成员。 FitzGerald 博士作为实验治疗中心主任和医学与工程研究所成员，与医学与工程研究所所长 Davies 博士和脂质诊所主任 Rader 博士有着广泛的互动；通过现有计划项目拨款和 SCOR 拨款担任普通临床研究中心助理主任。这些研究项目的另外三名研究人员（Eberwine、Ischiropoulos 和 Roos 博士）将需要在更有限的基础上使用该仪器。拟议的针对衰老这两种主要退行性疾病、癌症和心血管疾病的研究将涉及高度互动的方法。这将使一名研究人员取得的进展能够很容易地纳入其他研究人员的研究中。因此，虽然每个研究者的研究提案在科学价值和完整性方面都是独立的，但这个组合提案的附加价值是巨大的。