Neural circuitry of the parietal lobe

顶叶的神经回路

• 批准号: 6868828
• 负责人: RALPH M SIEGEL
• 金额: $ 14.16万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868828
• 关键词: Macaca mulatta; Sindbis virus; behavioral /social science research tag; bioimaging /biomedical imaging; biological models; brain imaging /visualization /scanning; cell morphology; ethology; fluorescence microscopy; fluorescent dye /probe; green fluorescent proteins; laboratory rat; neuroanatomy; neurons; parietal lobe /cortex; sensory cortex; single cell analysis; technology /technique development; transfection /expression vector

(Revised Abstract) DESCRIPTION This pilot research proposal is a conceptual break from a thirty year tradition characterizing the properties of single neurons in the inferior parietal lobule with increasingly complex behavioral tasks and analyses designed to isolate particular cognitive functions. Recent optical imaging studies have revealed the presence of multiple different topographic maps across the cortical surface of the inferior parietal lobule. The lowest resolution, and most robust is a representation of orbital gain fields; optic flow appears to be represented within this much as orientation is within retinotopy in V1. The proposal examines these two functions in inferior parietal lobe from a novel perspective- the neural circuit. There is simply no direct evidence in parietal cortex explaining how its sensory and gain field neuronal properties are generated. The molecular genetics of single neurons will be modified to measure the activity of selected neurons and populations of neurons. Standard microscopy will be used for population measures. Two-photon scanning microscopy will be use to directly compare cellular morphology and function. Specific neurons will be identified and studied over many days to assess the stability of their properties. This concept and the techniques for studying the circuitry is completely novel for association cortex; the use of modified molecular genetics of neurons in vivo in behaving animals has not been performed in any cortical area. The impact of these studies will be to open up cortex beyond striate in the behaving animal to circuit level analysis. In principle, the resolution of imaging can be extended to the level of the sub-cellular elements such as spines. In summary, this proposal is designed to understand properties of inferior parietal lobule neurons in terms of underlying neuronal circuits, much as investigators are now successfully exploring the circuit details of orientation tuning and color in primary visual cortex. Although the technical and conceptual issues are difficult, the expertise assembled is appropriate to the task. The successful outcome of these studies will be a substantial advance permitting direct visualization of the sub-cellular, cellular and circuit properties underlying the highest level cognitive functions such as attention, memory, intention and plasticity in the inferior parietal lobule.

（修订摘要）描述该试验研究建议是一个概念上的突破，从三十年的传统中，表征了下顶叶中单个神经元的特性，具有越来越复杂的行为任务和旨在隔离特定认知功能的分析。 最近的光学成像研究揭示了在下顶叶的皮质表面上存在多个不同的地形图。最低的分辨率，最健壮的是轨道增益场的表示。视值在此内似乎表示为在V1中的视网膜内。该提案从新的角度 - 神经回路检查了下顶叶中的这两个功能。顶叶皮层中根本没有直接证据，可以解释其感觉和增益场神经元特性的产生。 将修改单个神经元的分子遗传学，以测量所选神经元的活性。标准显微镜将用于人群度量。两光子扫描显微镜将用于直接比较细胞形态和功能。将在许多天中识别和研究特定的神经元，以评估其性质的稳定性。这个概念和研究电路的技术对于关联皮层来说是完全新颖的。在任何皮质区域尚未在体内使用修饰的神经元分子遗传学。这些研究的影响将是在行为动物的电路水平分析中打开超出条纹的皮质。原则上，成像的分辨率可以扩展到棘突等细胞元素的水平。总而言之，该提案旨在了解基础神经元电路的下叶叶叶神经元的特性，就像研究人员现在成功地探索了初级视觉皮层中的方向调整和颜色的电路细节一样。尽管技术和概念问题很困难，但组装的专业知识适合任务。这些研究的成功结果将是一个实质性的进步，允许直接可视化最高水平认知功能（例如下顶叶虫中的注意力，记忆，意图和可塑性）的亚细胞，细胞和电路特性。