Neural circuitry of the parietal lobe

顶叶的神经回路

• 批准号: 6868828
• 负责人: RALPH M SIEGEL
• 金额: $ 14.16万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868828
• 关键词: Macaca mulatta; Sindbis virus; behavioral /social science research tag; bioimaging /biomedical imaging; biological models; brain imaging /visualization /scanning; cell morphology; ethology; fluorescence microscopy; fluorescent dye /probe; green fluorescent proteins; laboratory rat; neuroanatomy; neurons; parietal lobe /cortex; sensory cortex; single cell analysis; technology /technique development; transfection /expression vector

(Revised Abstract) DESCRIPTION This pilot research proposal is a conceptual break from a thirty year tradition characterizing the properties of single neurons in the inferior parietal lobule with increasingly complex behavioral tasks and analyses designed to isolate particular cognitive functions. Recent optical imaging studies have revealed the presence of multiple different topographic maps across the cortical surface of the inferior parietal lobule. The lowest resolution, and most robust is a representation of orbital gain fields; optic flow appears to be represented within this much as orientation is within retinotopy in V1. The proposal examines these two functions in inferior parietal lobe from a novel perspective- the neural circuit. There is simply no direct evidence in parietal cortex explaining how its sensory and gain field neuronal properties are generated. The molecular genetics of single neurons will be modified to measure the activity of selected neurons and populations of neurons. Standard microscopy will be used for population measures. Two-photon scanning microscopy will be use to directly compare cellular morphology and function. Specific neurons will be identified and studied over many days to assess the stability of their properties. This concept and the techniques for studying the circuitry is completely novel for association cortex; the use of modified molecular genetics of neurons in vivo in behaving animals has not been performed in any cortical area. The impact of these studies will be to open up cortex beyond striate in the behaving animal to circuit level analysis. In principle, the resolution of imaging can be extended to the level of the sub-cellular elements such as spines. In summary, this proposal is designed to understand properties of inferior parietal lobule neurons in terms of underlying neuronal circuits, much as investigators are now successfully exploring the circuit details of orientation tuning and color in primary visual cortex. Although the technical and conceptual issues are difficult, the expertise assembled is appropriate to the task. The successful outcome of these studies will be a substantial advance permitting direct visualization of the sub-cellular, cellular and circuit properties underlying the highest level cognitive functions such as attention, memory, intention and plasticity in the inferior parietal lobule.

（修订摘要） 描述 这项试点研究提案是对三十年传统的概念突破，该传统描述​​了顶下小叶中单个神经元的特性，以及日益复杂的行为任务和旨在分离特定认知功能的分析。 最近的光学成像研究揭示了顶下小叶皮质表面存在多个不同的地形图。分辨率最低且最稳健的是轨道增益场的表示；光流似乎在这个范围内表示，因为方向在 V1 的视网膜局部内。该提案从一​​个新的角度——神经回路——研究了顶下叶的这两个功能。顶叶皮层根本没有直接证据来解释其感觉和增益场神经元特性是如何产生的。 单个神经元的分子遗传学将被修改以测量选定神经元和神经元群体的活动。标准显微镜将用于人口测量。双光子扫描显微镜将用于直接比较细胞形态和功能。特定的神经元将被识别并研究很多天，以评估其特性的稳定性。这个概念和研究电路的技术对于联合皮层来说是完全新颖的。尚未在任何皮质区域对行为动物体内的神经元进行改良分子遗传学的使用。这些研究的影响将是打开行为动物纹状体以外的皮层，以进行电路水平分析。原则上，成像的分辨率可以扩展到亚细胞元素（例如脊柱）的水平。总之，该提案旨在了解顶下小叶神经元在底层神经元回路方面的特性，就像研究人员现在正在成功探索初级视觉皮层中方向调节和颜色的回路细节一样。尽管技术和概念问题很困难，但所汇集的专业知识适合该任务。这些研究的成功结果将是一个重大进步，允许直接可视化最高水平认知功能（如顶下小叶的注意力、记忆、意图和可塑性）的亚细胞、细胞和回路特性。