NEURAL CIRCUITS OF THE INFERIOR PARIETAL LOBULE

顶下小叶的神经回路

• 批准号: 8171786
• 负责人: RALPH M SIEGEL
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171786
• 关键词: Behavior; Behavioral; Biological; Calcium; Computer Retrieval of Information on Scientific Projects Database; Data Set; Funding; Goals; Grant; Hour; Image; Inferior; Institution; Lobule; Measures; Microscopic; Monkeys; Neurons; Parietal; Pattern; Preparation; Research; Research Personnel; Resolution; Resources; Source; Surface; United States National Institutes of Health; Work; association cortex; in vivo; neural circuit; research study; sensor; spatial relationship; two-photon; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this proposal is to directly examine Mitchison and Cricks hypothesis in terms of the functional circuit organization of the inferior parietal lobule in behaving monkey. To study the functional circuit organization of inferior parietal lobule in vivo, we have spent the last three years perfecting the ability to perform two-photon imaging of genetically transformed neurons in the behaving monkey. These neurons are virally transfected to express a fluorescent sensor for voltage or calcium. Thus a neuron can be imaged at microscopic resolution along with its neuronal activity. Performing these experiments has demonstrated many advantages for the behaving monkey preparation. We have observed neurons a known depth from the pial surface, observed their precise spatial relationships to each other in the tangential plane, and measured their tuning during a behavioral task. Indeed, we have made stable continuous two-photon microscopic recordings from 15 neurons for 3 hours. These advances support examining specific projective functional circuits in association cortex during behavior. Our working hypothesis is: the inferior parietal lobule is organized into local modules containing neurons with the same projection patterns and the same functions. We will be using these funds to computationally analyze the large imaging data sets collected in the biological experiments.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该提案的目的是直接根据猴子下壁叶的功能电路组织直接检查米奇森和cricks假设。为了研究体内下顶叶的功能电路组织，我们已经花费了过去三年，完善了对行为猴子中遗传转化的神经元进行两光子成像的能力。这些神经元被病毒转染以表达电压或钙的荧光传感器。因此，神经元可以在显微镜分辨率及其神经元活性时成像。执行这些实验已经证明了行为猴子制备的许多优势。我们已经观察到神经元是从伴侣表面的已知深度，在切向平面上观察到它们的精确空间关系，并在行为任务中测量了它们的调整。实际上，我们已经从15个神经元中制作了稳定的连续两光子微观记录3小时。这些进步支持在行为过程中检查相关皮层中特定的投射功能电路。我们的工作假设是：下顶小叶群被组织到包含具有相同投影模式和相同功能的神经元的局部模块中。我们将使用这些资金来计算生物实验中收集的大型成像数据集。