Origins of T Helper Cell Function in Adaptive Immunity

适应性免疫中 T 辅助细胞功能的起源

基本信息

批准号：
6915628
负责人：
MICHAEL FREDERICK CRISCITIELLO
金额：
$ 4.83万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-07-07 至 2006-07-06
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6915628
关键词：
B lymphocyte T cell receptor T lymphocyte cellular immunity evolution haptens humoral immunity immunocytochemistry laboratory mouse laser capture microdissection leukocyte activation /transformation monoclonal antibody postdoctoral investigator sharks spleen

项目摘要

DESCRIPTION (provided by applicant): T lymphocyte function is central to the vertebrate adaptive immune response. Although all jawed vertebrates have the basic antigen receptors required, studies in sharks have suggested sub-optimal T cell function in this oldest such group compared to that of mammals. Recent data from the Flajnik lab suggest that the nurse shark may in fact have T cell zones in the spleenic white pulp. I have hypothesized that shark T cells do activate B cells in specific areas of secondary lymphoid tissue, thus displaying T cell anatomy and physiology akin to that of mammals. To test this hypothesis, I will identify the cells in the area adjacent to the B cell zones of the nurse shark spleen with immunohistochemistry. Functional assessment of T cell help will be conducted with hapten carrier conjugate experiments. In addition, laser-capture microdissection will further both the anatomical and functional aims with mapped TCR transcript analysis in primary and secondary lymphoid tissue. These studies will significantly advance our understanding of the phylogenetically fundamental aspects of adaptive cellular immunology, including T helper cell function and preclusion of T cell mediated autoimmunity. Concurrently, this work will provide the final step in preparation for my own independent program of research.

描述（由申请人提供）：T 淋巴细胞功能对于脊椎动物适应性免疫反应至关重要。尽管所有有颌脊椎动物都具有所需的基本抗原受体，但对鲨鱼的研究表明，与哺乳动物相比，这个最古老的此类群体的 T 细胞功能欠佳。 Flajnik 实验室的最新数据表明，护士鲨的脾白髓中实际上可能存在 T 细胞区域。我假设鲨鱼 T 细胞确实会激活次级淋巴组织特定区域的 B 细胞，从而显示出与哺乳动物相似的 T 细胞解剖学和生理学。为了验证这一假设，我将通过免疫组织化学鉴定护士鲨脾脏 B 细胞区附近区域的细胞。 T 细胞帮助的功能评估将通过半抗原载体缀合物实验进行。此外，激光捕获显微切割将通过初级和次级淋巴组织中的 TCR 转录图谱分析进一步实现解剖和功能目标。这些研究将显着增进我们对适应性细胞免疫学的系统发育基本方面的理解，包括 T 辅助细胞功能和 T 细胞介导的自身免疫的排除。同时，这项工作将为我自己的独立研究计划的准备提供最后一步。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Expressed IgH μ and τ transcripts share diversity segment in ranched Thunnus orientalis.

DOI：
10.1016/j.dci.2013.10.015
发表时间：
2014-03
期刊：
Developmental and comparative immunology
影响因子：
2.9
作者：
Mashoof S;Pohlenz C;Chen PL;Deiss TC;Gatlin D 3rd;Buentello A;Criscitiello MF
通讯作者：
Criscitiello MF

Fifty shades of immune defense.

DOI：
10.1371/journal.ppat.1003110
发表时间：
2013-02
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Criscitiello MF;de Figueiredo P
通讯作者：
de Figueiredo P

Expression of the inflammatory chemokines CCL2, CCL5 and CXCL2 and the receptors CCR1-3 and CXCR2 in T lymphocytes from mammary tumor-bearing mice.

DOI：
10.1016/j.cellimm.2011.05.004
发表时间：
2011
期刊：
CELLULAR IMMUNOLOGY
影响因子：
4.3
作者：
Owen, Jennifer L.;Criscitiello, Michael F.;Libreros, Stephania;Garcia-Areas, Ramon;Guthrie, Kathleen;Torroella-Kouri, Marta;Iragayarapu-Charyulu, Vijaya
通讯作者：
Iragayarapu-Charyulu, Vijaya

Distinct immunomodulatory effects of a panel of nanomaterials in human dermal fibroblasts.

DOI：
10.1016/j.toxlet.2012.01.022
发表时间：
2012-05
期刊：
Toxicology letters
影响因子：
3.5
作者：
A. Romoser;David E Figueroa;Aishwarya Sooresh;K. Scribner;Patricia L. Chen;W. Porter;M. Criscitiello;C. Sayes
通讯作者：
A. Romoser;David E Figueroa;Aishwarya Sooresh;K. Scribner;Patricia L. Chen;W. Porter;M. Criscitiello;C. Sayes