Analysis of Biomolecular Associations

生物分子关联分析

• 批准号: 6440234
• 负责人: Michael D. Brenowitz
• 金额: $ 27万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440234
• 关键词: CD1 molecule; DNA directed RNA polymerase; antiviral agents; binding sites; biomedical equipment purchase; gap junctions; glutathione transferase; human immunodeficiency virus; immunology; membrane channels; neurosciences; protein binding; protein protein interaction; proteomics; surface plasmon resonance; transferrin receptor; ultracentrifugation

DESCRIPTION (provided by the applicant): Ligand binding and macromolecular associations are generally grouped under the rubric "Reversible Associations" to denote their dependence upon noncovalent interactions that are in dynamic equilibrium in solution. A surface plasmon resonance instrument (BIAcore) is requested to join our existing analytical ultracentrifuge in order to establish a shared facility for the quantitative analysis of reversible associations. The research interests of the application participants encompass a broad range of important biomedical problems including assembly of gene transcription initiation and regulatory complexes, anti-HIV drug design, ligand binding and allostery, immunology and neuroscience. These instruments will be housed in a location convenient to the users' laboratories and will be accessible to the entire Einstein research community through the Laboratory for Macromolecular Analysis & Proteomics. The PI and other participants in this proposal have a proven track record in the organization and administration of shared resources. The institution provides substantial support to the Laboratory for Macromolecular Analysis & Proteomics including support for the instrument specialist who is overseeing use of the existing analytical ultracentrifuge and who will take charge of the new instrument requested in this grant.

描述（由申请人提供）：配体结合和大分子 关联通常分组在“可逆关联”标题下 表示它们对动态非共价相互作用的依赖 溶液中的平衡。表面等离子体共振仪（BIAcore） 要求加入我们现有的分析超速离心机，以便建立 用于可逆关联定量分析的共享工具。这 应用参与者的研究兴趣涵盖广泛 重要的生物医学问题，包括基因转录组装 启动和调节复合物、抗 HIV 药物设计、配体结合和 变构、免疫学和神经科学。这些仪器将安装在 位置方便，方便用户的实验室，并且可以方便地访问 整个爱因斯坦研究界通过大分子实验室 分析和蛋白质组学。 PI 和本提案的其他参与者有 在共享资源的组织和管理方面拥有良好的记录。 该机构为实验室提供大力支持 大分子分析和蛋白质组学，包括对仪器的支持 监督现有分析超速离心机的使用的专家 谁将负责这笔赠款中要求的新文书。