DNA-based information storage based on surface immobilised DNA

基于表面固定化DNA的DNA信息存储

• 批准号: 2599290
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2599290
• 关键词: DNA; based; information; storage; surface

The exponential increase in digital data requires fundamentally new technology for long-term data archiving. While the concept of using DNA for non-volatile storage was introduced almost 25 years ago, only recently have advances in DNA synthesis and sequencing made such nucleic acid memories (NAMs) a viable proposition. However, at present, no end-to-end solution that can be integrated into existing silicon, IT technology exists. This project will investigate an end-to-end, hybrid semiconductor-NAM where information can be stored and processed in both the electronic and molecular domains and can move freely across the interface between domains. This project will focus on two challenges that underpin this vision: 1) design and optimisation of a DNA memory based on a library of DNA hairpins; 2) experimental demonstration of electronic query of the hairpin NAM through the use of pH-dependent DNA switches to electronically actuate key-based searching. This will be a highly experimental project using a range of techniques common to DNA nanotechnology including fluorescence and circular dichroism to explore DNA hairpin opening and quartz crystal microbalance with dissipation monitoring and TIRF microscopy to explore triplex switching as a function of pH. This programme is interdisciplinary with the potential for impact across multiple EPSRC themes, including Information and communication technologies, Microelectronics device technology and Biophysics and soft matter physics. Additionally, hybrid electronic-DNA technologies have particular relevance in medical diagnostics e.g., DNA aptamer-based biosensors. This project thus also contributes indirectly to the EPSRC Healthcare Technologies theme and the EPSRC Grand Challenge Understanding the Physics of Life.

数字数据的指数级增长需要全新的长期数据归档技术。虽然使用 DNA 进行非易失性存储的概念在大约 25 年前就已提出，但直到最近 DNA 合成和测序方面的进步才使这种核酸存储器 (NAM) 成为可行的主张。然而，目前不存在可以集成到现有芯片、IT 技术中的端到端解决方案。该项目将研究一种端到端的混合半导体 NAM，其中信息可以在电子域和分子域中存储和处理，并且可以在域之间的界面上自由移动。该项目将重点关注支撑这一愿景的两个挑战：1）基于 DNA 发夹库的 DNA 存储器的设计和优化； 2) 通过使用pH依赖性DNA开关以电子方式驱动基于键的搜索来对发夹NAM进行电子查询的实验演示。这将是一个高度实验性的项目，使用 DNA 纳米技术常见的一系列技术，包括荧光和圆二色性来探索 DNA 发夹开口和石英晶体微天平，并通过耗散监测和 TIRF 显微镜来探索三重开关作为 pH 的函数。该项目是跨学科的，有可能对多个 EPSRC 主题产生影响，包括信息和通信技术、微电子设备技术以及生物物理学和软物质物理学。此外，混合电子 DNA 技术在医学诊断中具有特别的相关性，例如基于 DNA 适体的生物传感器。因此，该项目也间接为 EPSRC 医疗保健技术主题和 EPSRC 了解生命物理学​​大挑战做出了贡献。