CORE--INFRASTRUCTURE AND CRYO-EM RESOURCE DEVELOPMENT

核心——基础设施和冷冻电镜资源开发

• 批准号: 6757139
• 负责人: ROBERT M GLAESER
• 金额: $ 18.25万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6757139
• 关键词: automated data processing; bioimaging /biomedical imaging; biomedical facility; biotechnology; computer data analysis; computer graphics /printing; computer program /software; cryoelectron microscopy; image processing; macromolecule; nanotechnology; parallel processing

The Core Project consists of two sub-projects, both of which support the objectives of the other Projects within the Program as a whole. In Project F1 we propose to acquire test-image data-sets for ice-embedded single particles, which will be archived in a way that is available simultaneously to the software developers in this program project and to the community via the web. The known atomic structures of these specimens will provide a reality check for validating our computational methodology. The broader availability of the data sets should further stimulate new algorithmic development towards atomic structure determination by electron cryomicroscopy of single particles. Our primary test molecules will be GroEL particles and tubulin (in the form of microtubules), both of which are chosen because of their known atomic structure, appropriate size and inherent symmetry. We will obtain focal pairs of images for more than 300,000 single-particles of each type of sample. Images will be recorded on the JEOL3000 field emission electron cryo-microscopes (equipped with a liquid helium cryo-stage) located in Houston and in Berkeley. in addition to the labor-intensive data recording, we expect to be involved in improving the methodology of specimen preparation and efficient data-recording. Project F2 will provide key scientific infrastructure that is needed within the Program. Project F2 will also provide local scientific expertise in cryo-EM that is needed to support the work of Projects C and D, for which the primary expertise of the PIs lies in computer science and mathematics, respectively. Finally, Project F2 will coordinate a range of centralized functions that are related to efficient communication and collaboration between all Projects.

核心项目由两个子项目组成，两者都支持另一个项目的目标 整个程序中的项目。 在Project F1中，我们建议为冰嵌入的单个粒子获取测试图像数据集，该数据集将以同时归档该程序项目中的软件开发人员以及通过Web的社区来归档。这些标本的已知原子结构将提供现实检查，以验证我们的计算方法。数据集的更广泛的可用性应进一步刺激单个颗粒的电子冻伤测定原子结构的新算法发展。我们的主要测试分子将是凹槽颗粒和微管蛋白（以微管的形式），它们是由于已知的原子结构，适当的大小和固有的对称性而选择的。我们将获得每种样品的300,000多个单粒子的图像对焦对。图像将记录在 JEOL3000田间发射电子冷冻微镜（配备了位于休斯敦和伯克利的液体氦气级别阶段）。除了劳动密集型数据记录外，我们还希望将参与改进标本制备和有效数据录制的方法。 F2项目将提供计划中需要的关键科学基础架构。 F2项目还将提供Cryo-EM的本地科学专业知识，以支持C和D项目的工作，PIS的主要专业知识分别在于计算机科学和数学。最后，F2项目将协调一系列与所有项目之间有效的沟通和协作有关的集中式功能。