Cell Signaling in Meiosis

减数分裂中的细胞信号转导

基本信息

批准号：
6915762
负责人：
JOANNE ENGEBRECHT
金额：
$ 25.99万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-07-01 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6915762
关键词：
Saccharomyces cerevisiae biological signal transduction cell differentiation cytogenetics developmental genetics electron microscopy fungal genetics green fluorescent proteins meiosis membrane activity membrane permeability phospholipase D protein kinase protein structure function sporogenesis

项目摘要

DESCRIPTION (provided by applicant): Gametogenesis is essential for the propagation of all sexually reproducing organisms and consists of halving the chromosome number through meiosis, and the subsequent packaging of the haploid products into gametes (sperm and eggs in mammals). Meiosis and gamete formation must be tightly coupled to ensure the formation of viable progeny; perturbations result in infertility, inviability and birth defects. Not surprisingly, a large number of signaling molecules participate in precisely regulating this cellular differentiation program. In the yeast Saccharomyces cerevisiae, sexual reproduction occurs via sporulation and is similar in many respects to gametogenesis in mammals. We have shown that the conserved signaling molecules Phospholipase D (PLO) and the Ste2O-like kinase, Sps1 are essential for yeast sporulation, but their precise roles in this process are largely unknown.We propose to continue to exploit the genetics of yeast to elucidate the signaling pathways involved in regulating the cellular differentiation program of sporulation. We will analyze the role of PLO and Sps1 using a three-pronged approach: 1) we wilt investigate the specific requirement for PLO-generated lipids in sporulation. 2) We will elucidate the function of a developmentally regulated Art-GAP and determine if it mediates PLO pathway function. 3) We will examine the relationship between PLD and Sps1 and identify additional components of the Sps1 signaling pathway. Taken together, our studies will provide a framework for understanding how cell signaling events are coordinated to ensure the formation of viable progeny. Given the extensive degree of evolutionary conservation between these molecules, it is likely that an understanding of the function of PLD and Sps1 in yeast wilt provide general insights into the role of orthologous molecules in humans and the consequences of their altered regulation in infertility, birth defects and disease.

描述（由申请人提供）：配子发生对于所有性繁殖生物的传播至关重要，包括通过减数分裂将染色体数量减半，以及随后将单倍体产物包装到配子中（哺乳动物中的精子和卵）。减数分裂和配子的形成必须紧密耦合，以确保形成可行的后代；扰动导致不孕，不可分割和出生缺陷。毫不奇怪，大量信号分子参与了该细胞分化程序的精确调节。在酿酒酵母的酵母菌中，有性繁殖是通过孢子形成发生的，并且在许多方面与哺乳动物的配子发生相似。我们已经表明，保守的信号分子磷脂酶D（PLO）和类似Ste2O的激酶SPS1对于酵母菌孢子形成至关重要，但是它们在此过程中的精确作用在很大程度上是未知的。我们建议继续利用酵母的遗传学，以阐明涉及散布细胞分化的信号传导范围。我们将使用三方面的方法分析PLO和SPS1的作用：1）我们枯萎研究了孢子形成plo生成的脂质的特定要求。 2）我们将阐明受发展调控的ART-差距的功能，并确定它是否介导PLO途径函数。 3）我们将检查PLD和SPS1之间的关系，并确定SPS1信号通路的其他组件。综上所述，我们的研究将提供一个框架，以了解细胞信号事件如何协调以确保形成可行的后代。鉴于这些分子之间的广泛进化保守程度，对PLD和SPS1在酵母菌中的功能的理解很可能提供了对直系同源分子在人类中的作用的一般见解，以及其不育调节在不育，出生缺陷和疾病中改变的后果。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways.

在酵母孢子形成过程中，Snc1p v-SNARE 向原生孢子膜的运输依赖于内体修复途径。

DOI：
10.1111/j.1600-0854.2007.00606.x
发表时间：
2007
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
0
作者：
Morishita,Masayo;Mendonsa,Rima;Wright,Jennifer;Engebrecht,Joanne
通讯作者：
Engebrecht,Joanne

Roles of phosphoinositides and of Spo14p (phospholipase D)-generated phosphatidic acid during yeast sporulation.

磷酸肌醇和 Spo14p（磷脂酶 D）生成的磷脂酸在酵母孢子形成过程中的作用。

DOI：
10.1091/mbc.e03-04-0245
发表时间：
2004
期刊：
Molecular biology of the cell
影响因子：
3.3
作者：
Rudge,SimonA;Sciorra,VickiA;Iwamoto,Michelle;Zhou,Chun;Strahl,Thomas;Morris,AndrewJ;Thorner,Jeremy;Engebrecht,JoAnne
通讯作者：
Engebrecht,JoAnne

The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.

Arf-GTP 酶激活蛋白 Gcs1p 对于孢子形成至关重要，并调节磷脂酶 D Spo14p。

DOI：
10.1128/ec.5.1.112-124.2006
发表时间：
2006
期刊：
Eukaryotic cell.
影响因子：
0
作者：
Connolly,JaimeE;Engebrecht,Joanne
通讯作者：
Engebrecht,Joanne

Dual role for phosphoinositides in regulation of yeast and mammalian phospholipase D enzymes.

DOI：
10.1083/jcb.200205056
发表时间：
2002-12-23
期刊：
JOURNAL OF CELL BIOLOGY
影响因子：
7.8
作者：
Sciorra, Vicki A;Rudge, Simon A;Wang, Jiyao;McLaughlin, Stuart;Engebrecht, JoAnne;Morris, Andrew J
通讯作者：
Morris, Andrew J

Sorting signals within the Saccharomyces cerevisiae sporulation-specific dityrosine transporter, Dtr1p, C terminus promote Golgi-to-prospore membrane transport.

酿酒酵母孢子形成特异性二酪氨酸转运蛋白、Dtr1p、C 末端内的分选信号促进高尔基体到原生孢子膜的转运。